Literature DB >> 1711990

Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.

R A Bowman1, G L O'Neill, T V Riley.   

Abstract

A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemiluminescence (ECL) gene detection system. The probe labelling procedure was easy to perform, taking only 20 min. The complete typing method was comparatively simple, reproducible and readily adaptable to most bacterial genera.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1711990     DOI： 10.1016/0378-1097(91)90097-t

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  10 in total

1.  Genetic diversity within isolates of mutans streptococci recognized by an rRNA gene probe.

Authors:  M Saarela; S Alaluusua; T Takei; S Asikainen
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

2.  Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

Authors:  P Bidet; V Lalande; B Salauze; B Burghoffer; V Avesani; M Delmée; A Rossier; F Barbut; J C Petit
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

3.  Identity of streptococcal blood isolates and oral isolates from two patients with infective endocarditis.

Authors:  N E Fiehn; E Gutschik; T Larsen; J M Bangsborg
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

4.  Usefulness of culture in the diagnosis of Clostridium difficile infection.

Authors:  F Bond; G Payne; S P Borriello; H Humphreys
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-03       Impact factor: 3.267

5.  Comparison of typing methods for Clostridium difficile isolates.

Authors:  M J Wolfhagen; A C Fluit; R Torensma; M Jansze; A F Kuypers; E A Verhage; J Verhoef
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

6.  Comparison of restriction enzyme analysis, arbitrarily primed PCR, and protein profile analysis typing for epidemiologic investigation of an ongoing Clostridium difficile outbreak.

Authors:  M E Rafferty; A L Baltch; R P Smith; L H Bopp; C Rheal; F C Tenover; G E Killgore; D M Lyerly; T D Wilkins; D J Schoonmaker; G E Hannett; M Shayegani
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

7.  A molecular characterization of Clostridium difficile isolates from humans, animals and their environments.

Authors:  G O'Neill; J E Adams; R A Bowman; T V Riley
Journal:  Epidemiol Infect       Date:  1993-10       Impact factor: 2.451

8.  Investigation of an outbreak of Clostridium difficile infection in a general hospital by numerical analysis of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  M Costas; B Holmes; S L On; M Ganner; M C Kelly; S K Nath
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

Review 9.  Epidemiological aspects of infections caused by Bacteroides fragilis and Clostridium difficile.

Authors:  S Tabaqchali; M Wilks
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-11       Impact factor: 3.267

10.  Identification of outbreak-associated and other strains of Clostridium difficile by numerical analysis of SDS-PAGE protein patterns.

Authors:  M Costas; B Holmes; M Ganner; S L On; P N Hoffman; M A Worsley; H Panigrahi
Journal:  Epidemiol Infect       Date:  1994-08       Impact factor: 2.451
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.