OBJECTIVE: The aim was to develop a more efficient molecular detection system than histological examination (HE) for lymph node (LN) metastasis. METHODS: Cytokeratin (CK) 19 mRNA copy numbers of 5 colon carcinoma cell lines (Lovo, DLD1, WiDr, Colo201 and Colo320) were calculated and compared by one-step nucleic acid amplification (OSNA) and conventional real-time reverse-transcription polymerase chain reaction (RT-PCR). Then, 91 LN submitted for HE from 6 patients with advanced colorectal adenocarcinoma and 64 LN submitted for frozen diagnosis from 47 patients with different malignancies were examined by OSNA and HE. RESULTS: CK19 mRNA copy numbers of all but Colo320 cells detected by OSNA were within double of those detected by RT-PCR. The least cell count of Lovo cells detected at one reaction (2 microl) by OSNA was calculated as 0.8 cells. Carcinoma metastasis showing either HE+ or OSNA+ was detected in 7.9% of the LN from advanced colorectal adenocarcinomas and in 30.0% of the LN for frozen diagnosis from different malignancies; HE-/OSNA+ metastasis was detected in 4.8 and 4.0%, respectively. OSNA analysis of 1 LN could be completed within 40 min. CONCLUSION: A combined analysis of LN by HE and OSNA could increase the sensitivity for detecting micrometastasis during surgery.
OBJECTIVE: The aim was to develop a more efficient molecular detection system than histological examination (HE) for lymph node (LN) metastasis. METHODS: Cytokeratin (CK) 19 mRNA copy numbers of 5 colon carcinoma cell lines (Lovo, DLD1, WiDr, Colo201 and Colo320) were calculated and compared by one-step nucleic acid amplification (OSNA) and conventional real-time reverse-transcription polymerase chain reaction (RT-PCR). Then, 91 LN submitted for HE from 6 patients with advanced colorectal adenocarcinoma and 64 LN submitted for frozen diagnosis from 47 patients with different malignancies were examined by OSNA and HE. RESULTS:CK19 mRNA copy numbers of all but Colo320 cells detected by OSNA were within double of those detected by RT-PCR. The least cell count of Lovo cells detected at one reaction (2 microl) by OSNA was calculated as 0.8 cells. Carcinoma metastasis showing either HE+ or OSNA+ was detected in 7.9% of the LN from advanced colorectal adenocarcinomas and in 30.0% of the LN for frozen diagnosis from different malignancies; HE-/OSNA+ metastasis was detected in 4.8 and 4.0%, respectively. OSNA analysis of 1 LN could be completed within 40 min. CONCLUSION: A combined analysis of LN by HE and OSNA could increase the sensitivity for detecting micrometastasis during surgery.
Authors: Christian Schem; Nicolai Maass; Dirk O Bauerschlag; Martin H Carstensen; Thomas Löning; Christian Roder; Olivera Batic; Walter Jonat; Katharina Tiemann Journal: Virchows Arch Date: 2008-12-20 Impact factor: 4.064
Authors: Roland S Croner; Vera Schellerer; Helene Demund; Claus Schildberg; Thomas Papadopulos; Elisabeth Naschberger; Michael Stürzl; Klaus E Matzel; Werner Hohenberger; Anne Schlabrakowski Journal: J Transl Med Date: 2010-09-06 Impact factor: 5.531
Authors: Benjamin Weixler; Sofia Teixeira da Cunha; René Warschkow; Nicolas Demartines; Ulrich Güller; Andreas Zettl; Alexander Vahrmeijer; Cornelis J H van de Velde; Carsten T Viehl; Markus Zuber Journal: World J Surg Date: 2021-01-29 Impact factor: 3.352
Authors: Mike Visser; Mehdi Jiwa; Anja Horstman; Antoinette A T P Brink; Rene P Pol; Paul van Diest; Peter J F Snijders; Chris J L M Meijer Journal: Int J Cancer Date: 2008-06-01 Impact factor: 7.396