Literature DB >> 1711872

A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures.

W Kedzierski1, J C Porter.   

Abstract

A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.

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Year:  1991        PMID： 1711872

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  14 in total

1.  Expression of the woodchuck N-myc2 retroposon in brain and in liver tumors is driven by a cryptic N-myc promoter.

Authors:  G Fourel; C Transy; B C Tennant; M A Buendia
Journal:  Mol Cell Biol       Date:  1992-12       Impact factor: 4.272

2.  CpG motifs in bacterial DNA cause inflammation in the lower respiratory tract.

Authors:  D A Schwartz; T J Quinn; P S Thorne; S Sayeed; A K Yi; A M Krieg
Journal:  J Clin Invest       Date:  1997-07-01       Impact factor: 14.808

3.  Effect of alpha interferon on the hepatitis C virus replicon.

Authors:  J T Guo; V V Bichko; C Seeger
Journal:  J Virol       Date:  2001-09       Impact factor: 5.103

4.  The HNF1/HNF4-dependent We2 element of woodchuck hepatitis virus controls viral replication and can activate the N-myc2 promoter.

Authors:  G Fourel; F Ringeisen; M Flajolet; F Tronche; M Pontoglio; P Tiollais; M A Buendia
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

5.  VPg Northern-immunoblots as a means for detection of viral RNAs in protoplasts or plants infected with grapevine fanleaf nepovirus.

Authors:  R Margis; F Hans; L Pinck
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

6.  Polyoma virus early-late switch: regulation of late RNA accumulation by DNA replication.

Authors:  Z Liu; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-15       Impact factor: 11.205

7.  Hepadnavirus integration: mechanisms of activation of the N-myc2 retrotransposon in woodchuck liver tumors.

Authors:  Y Wei; G Fourel; A Ponzetto; M Silvestro; P Tiollais; M A Buendia
Journal:  J Virol       Date:  1992-09       Impact factor: 5.103

8.  Sequence-selective carbohydrate-DNA interaction: dimeric and monomeric forms of the calicheamicin oligosaccharide interfere with transcription factor function.

Authors:  C Liu; B M Smith; K Ajito; H Komatsu; L Gomez-Paloma; T Li; E A Theodorakis; K C Nicolaou; P K Vogt
Journal:  Proc Natl Acad Sci U S A       Date:  1996-01-23       Impact factor: 11.205

9.  Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.

Authors:  K K Young; R M Resnick; T W Myers
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

10.  A single element of the elastase I enhancer is sufficient to direct transcription selectively to the pancreas and gut.

Authors:  S D Rose; F Kruse; G H Swift; R J MacDonald; R E Hammer
Journal:  Mol Cell Biol       Date:  1994-03       Impact factor: 4.272
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