| Literature DB >> 17115035 |
Arne Hansen1, Yidong Chen, Jason M Inman, Quang N Phan, Zhi-Qing Qi, Charlie C Xiang, Miklos Palkovits, Natasha Cherman, Sergei A Kuznetsov, Pamela G Robey, Eva Mezey, Michael J Brownstein.
Abstract
G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.Entities:
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Year: 2006 PMID： 17115035 DOI： 10.1038/nmeth977
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547