| Literature DB >> 17113868 |
Lijuan Zhang1, Roger Lawrence, Beth A Frazier, Jeffrey D Esko.
Abstract
Most glycosaminoglycan (GAG)-defective mutants have been isolated and characterized from Chinese hamster ovary (CHO) cells. Wild-type and GAG-defective CHO cells have been used by several hundreds of laboratories to study how altering the GAG structure of proteoglycans affects fundamental properties of cells, such as bacterial/viral infection, signaling, protein degradation, and cell adhesion. This chapter describes methods used to construct and characterize new CHO cell lines with gain-of-function GAG structures. These novel CHO cell lines allow herpes simplex virus (HSV) entry or have anticoagulant properties that are not possessed by wild-type CHO cells. The method used to study GAG biosynthetic mechanisms that control specific GAG sequence assembly is also described.Entities:
Mesh：
Substances：
Year: 2006 PMID： 17113868 DOI： 10.1016/S0076-6879(06)16013-9
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600