Literature DB >> 1711232

Identification of an immunodominant epitope within the capsid protein of hepatitis C virus.

M S Nasoff1, S L Zebedee, G Inchauspé, A M Prince.   

Abstract

We have isolated cDNA clones from the 5' end of the Hutchinson strain of hepatitis C virus. Sequences encoding various segments of the HCV structural region were fused to the gene for glutathione S-transferase and analyzed for the expression of hepatitis C virus-capsid fusion proteins. With a set of these fusion proteins, both human and chimpanzee immune responses to capsid were studied. An immunodominant epitope was located within the amino-terminal portion of capsid that is preferentially recognized by antibodies in both human and chimpanzee hepatitis C virus-positive sera. In addition, analyses of sequential serum samples taken from humans and chimpanzees with either chronic or apparently self-limited infections revealed that a strong anti-capsid response develops rapidly after onset of infection.

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Year:  1991        PMID： 1711232      PMCID： PMC51893          DOI： 10.1073/pnas.88.12.5462

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

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2.  Use of a chemically modified T7 DNA polymerase for manual and automated sequencing of supercoiled DNA.

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3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Antibody reactivity to different regions of human T-cell leukemia virus type 1 gp61 in infected people.

Authors:  Y M Chen; T H Lee; K P Samuel; A Okayama; N Tachibana; I Miyoshi; T S Papas; M Essex
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

5.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

6.  Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis.

Authors:  H J Alter; R H Purcell; J W Shih; J C Melpolder; M Houghton; Q L Choo; G Kuo
Journal:  N Engl J Med       Date:  1989-11-30       Impact factor: 91.245

7.  An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.

Authors:  G Kuo; Q L Choo; H J Alter; G L Gitnick; A G Redeker; R H Purcell; T Miyamura; J L Dienstag; M J Alter; C E Stevens
Journal:  Science       Date:  1989-04-21       Impact factor: 47.728

8.  Hepatitis C virus antibodies in chronic active hepatitis: pathogenetic factor or false-positive result?

Authors:  I G McFarlane; H M Smith; P J Johnson; G P Bray; D Vergani; R Williams
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9.  Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region.

Authors:  H Okamoto; S Okada; Y Sugiyama; T Tanaka; Y Sugai; Y Akahane; A Machida; S Mishiro; H Yoshizawa; Y Miyakawa
Journal:  Jpn J Exp Med       Date:  1990-08

10.  Enzyme-linked immunosorbent assay for antibodies against the capsid protein of hepatitis C virus with a synthetic oligopeptide.

Authors:  H Okamoto; E Munekata; F Tsuda; K Takahashi; S Yotsumoto; T Tanaka; K Tachibana; Y Akahane; Y Sugai; Y Miyakawa
Journal:  Jpn J Exp Med       Date:  1990-08
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  22 in total

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Authors:  P Ferroni; G Mascolo; M Zaninetti; D Colzani; F Pregliasco; M Pirisi; F Barbone; V Gasparini
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3.  Correlation of serum antibody titers against hepatitis C virus core protein with clinical features by western blot (immunoblot) analysis using a recombinant vaccinia virus expression system.

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5.  Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein. Detection of anti-hepatitis C core antibody by western blot.

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6.  Immunodominant regions within the hepatitis C virus core and putative matrix proteins.

Authors:  M Sällberg; U Rudén; B Wahren; L O Magnius
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7.  Hepatitis C virus (HCV)-specific cytotoxic T lymphocytes recognize epitopes in the core and envelope proteins of HCV.

Authors:  M J Koziel; D Dudley; N Afdhal; Q L Choo; M Houghton; R Ralston; B D Walker
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8.  Isotype-specific immune response to a single hepatitis C virus core epitope defined by a human monoclonal antibody: diagnostic value and correlation to PCR.

Authors:  K Siemoneit; M da Silva Cardoso; A Wölpl; S Epple; H Wintersinger; K Koerner; B Kubanek
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9.  Chimeric hepatitis B virus core particles with parts or copies of the hepatitis C virus core protein.

Authors:  A Yoshikawa; T Tanaka; Y Hoshi; N Kato; K Tachibana; H Iizuka; A Machida; H Okamoto; M Yamasaki; Y Miyakawa
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10.  Interaction of immune sera with synthetic peptides corresponding to the structural protein region of hepatitis C virus.

Authors:  W M Ching; C Wychowski; M J Beach; H Wang; C L Davies; M Carl; D W Bradley; H J Alter; S M Feinstone; J W Shih
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205
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