Literature DB >> 17110214

Digital autofocus methods for automated microscopy.

Feimo Shen1, Louis Hodgson, Klaus Hahn.   

Abstract

Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.

Mesh:

Year:  2006        PMID: 17110214     DOI: 10.1016/S0076-6879(06)14032-X

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN: 0076-6879            Impact factor:   1.600


  10 in total

1.  Biosensors for characterizing the dynamics of rho family GTPases in living cells.

Authors:  Louis Hodgson; Feimo Shen; Klaus Hahn
Journal:  Curr Protoc Cell Biol       Date:  2010-03

2.  Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.

Authors:  Zhaojun Wang; Ming Lei; Baoli Yao; Yanan Cai; Yansheng Liang; Yanlong Yang; Xibin Yang; Hui Li; Daxi Xiong
Journal:  Biomed Opt Express       Date:  2015-10-14       Impact factor: 3.732

3.  Quantitative automated image analysis system with automated debris filtering for the detection of breast carcinoma cells.

Authors:  David T Martin; Sergio Sandoval; Casey N Ta; Manuel E Ruidiaz; Maria Jose Cortes-Mateos; Davorka Messmer; Andrew C Kummel; Sarah L Blair; Jessica Wang-Rodriguez
Journal:  Acta Cytol       Date:  2011-04-27       Impact factor: 2.319

4.  Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning.

Authors:  Chen Li; Mani Ratnam Rai; H Troy Ghashghaei; Alon Greenbaum
Journal:  Biomed Opt Express       Date:  2022-01-21       Impact factor: 3.732

5.  A biosensor of S100A4 metastasis factor activation: inhibitor screening and cellular activation dynamics.

Authors:  Sarah C Garrett; Louis Hodgson; Andrew Rybin; Alexei Toutchkine; Klaus M Hahn; David S Lawrence; Anne R Bresnick
Journal:  Biochemistry       Date:  2007-12-23       Impact factor: 3.162

Review 6.  Automated microscopy for high-content RNAi screening.

Authors:  Christian Conrad; Daniel W Gerlich
Journal:  J Cell Biol       Date:  2010-02-22       Impact factor: 10.539

7.  DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms.

Authors:  Kevin D Healy; Louis Hodgson; Tai-Young Kim; Adam Shutes; Savitri Maddileti; Rudolph L Juliano; Klaus M Hahn; T Kendall Harden; Yung-Jue Bang; Channing J Der
Journal:  Mol Carcinog       Date:  2008-05       Impact factor: 4.784

8.  Automated Remote Focusing, Drift Correction, and Photostimulation to Evaluate Structural Plasticity in Dendritic Spines.

Authors:  Michael S Smirnov; Paul R Evans; Tavita R Garrett; Long Yan; Ryohei Yasuda
Journal:  PLoS One       Date:  2017-01-23       Impact factor: 3.240

Review 9.  Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.

Authors:  Tal M Dankovich; Silvio O Rizzoli
Journal:  iScience       Date:  2021-02-03

10.  Light microscopy applications in systems biology: opportunities and challenges.

Authors:  Paul Michel Aloyse Antony; Christophe Trefois; Aleksandar Stojanovic; Aidos Sagatovich Baumuratov; Karol Kozak
Journal:  Cell Commun Signal       Date:  2013-04-11       Impact factor: 5.712

  10 in total

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