| Literature DB >> 17110214 |
Feimo Shen1, Louis Hodgson, Klaus Hahn.
Abstract
Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.Mesh:
Year: 2006 PMID: 17110214 DOI: 10.1016/S0076-6879(06)14032-X
Source DB: PubMed Journal: Methods Enzymol ISSN: 0076-6879 Impact factor: 1.600