Literature DB >> 17109500

Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay.

Kuo-Ching Yang1, Alexander Chu, Chao-Sheng Liao, Yu-Min Lin, Gen-Min Wang.   

Abstract

AIM: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer.
METHODS: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore).
RESULTS: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference.
CONCLUSION: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.

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Year:  2006        PMID: 17109500      PMCID: PMC4087349          DOI: 10.3748/wjg.v12.i43.7029

Source DB:  PubMed          Journal:  World J Gastroenterol        ISSN: 1007-9327            Impact factor:   5.742


  28 in total

1.  IgG immune response to Helicobacter pylori antigens in patients with gastric cancer as defined by ELISA and immunoblotting.

Authors:  K Klaamas; M Held; T Wadström; A Lipping; O Kurtenkov
Journal:  Int J Cancer       Date:  1996-07-03       Impact factor: 7.396

2.  Helicobacter pylori in tumor tissues of patients with advanced gastric adenocarcinoma: high prevalence but failure to detect integration.

Authors:  J T Wang; C T Sung; J T Lin; T H Wang
Journal:  Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi       Date:  1996-08

Review 3.  Helicobacter pylori infection and precancerous lesions of the stomach.

Authors:  J Valle; J P Gisbert
Journal:  Hepatogastroenterology       Date:  2001 Nov-Dec

4.  Antibody response of patients with Helicobacter pylori-related gastric adenocarcinoma: significance of anti-cagA antibodies.

Authors:  C Vaucher; B Janvier; J B Nousbaum; B Grignon; L Pezennec; M Robaszkiewicz; H Gouerou; B Picard; J L Fauchere
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

5.  Positive serum antibody and negative tissue staining for Helicobacter pylori in subjects with atrophic body gastritis.

Authors:  W E Karnes; I M Samloff; M Siurala; M Kekki; P Sipponen; S W Kim; J H Walsh
Journal:  Gastroenterology       Date:  1991-07       Impact factor: 22.682

6.  Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique.

Authors:  L P Andersen; F Espersen
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

7.  Immunoblot assay for serodiagnosis of Helicobacter pylori infections.

Authors:  I Nilsson; A Ljungh; P Aleljung; T Wadström
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

8.  Antibodies to CagA protein are associated with gastric atrophy in Helicobacter pylori infection.

Authors:  I L Beales; J E Crabtree; D Scunes; A Covacci; J Calam
Journal:  Eur J Gastroenterol Hepatol       Date:  1996-07       Impact factor: 2.566

9.  Search for putative virulence factors of Helicobacter pylori: the low-molecular-weight (33-35 K) antigen.

Authors:  Y Yamaoka; T Kodama; D Y Graham; K Kashima
Journal:  Dig Dis Sci       Date:  1998-07       Impact factor: 3.199

10.  Helicobacter pylori infection and the risk of gastric carcinoma.

Authors:  J Parsonnet; G D Friedman; D P Vandersteen; Y Chang; J H Vogelman; N Orentreich; R K Sibley
Journal:  N Engl J Med       Date:  1991-10-17       Impact factor: 91.245

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  1 in total

1.  Evaluation of Invasive and Noninvasive Methods for the Diagnosis of Helicobacter Pylori Infection

Authors:  Yasemin Cosgun; Abdullah Yildirim; Mihriban Yucel; Ayse Esra Karakoc; Gokhan Koca; Alpaslan Gonultas; Gul Gursoy; Huseyin Ustun; Meliha Korkmaz
Journal:  Asian Pac J Cancer Prev       Date:  2016-12-01
  1 in total

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