| Literature DB >> 17107658 |
Satoru Watanabe1, Toshiaki Kobayashi, Masakazu Saito, Masumi Sato, Kaori Nimura-Matsune, Taku Chibazakura, Shigeru Taketani, Hitoshi Nakamoto, Hirofumi Yoshikawa.
Abstract
In cyanobacterium Synechococcus elongatus PCC 7942, we observed that htpG-overexpression caused remarkable growth inhibition. In addition, subcellular fractionation experiments showed that HtpG was localized in the membrane fraction. To understand its function in cyanobacteria, we carried out yeast two-hybrid screening to identify specific proteins interacting with HtpG, and found out, HemE, uroporphyrinogen decarboxylase. When compared to the wild-type strain, the htpG-null mutant and -overexpressing strains exhibited higher and lower cytosolic HemE activity, based on the coproporphyrin production, respectively. These results strongly suggest that HtpG is involved in the regulation of tetrapyrrole biosynthesis through interacting with HemE protein.Entities:
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Year: 2006 PMID： 17107658 DOI： 10.1016/j.bbrc.2006.10.144
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575