Literature DB >> 17105946

The comet assay as a method of assessment of neurotoxicity: usefulness for drugs of abuse.

Giada Frenzilli1, Vittoria Scarcelli, Francesco Fornai, Antonio Paparelli, Marco Nigro.   

Abstract

Comet assay is a quick and versatile method for assessing DNA damage in individual cells. It allows the detection of single and double DNA strand breaks, as well as the presence of alkali labile sites. DNA breaks may represent the direct effect of some damaging agent, or they may be intermediates in cellular repair. DNA strand breaks may also come from the action of free radicals generated by oxidative stress processes. The present article summarizes some data from our and other groups underlining the suitability of the Comet assay in assessing neurotoxicity and its potential in evaluating drugs of abuse-related genotoxicity.

Entities:  

Mesh：

Year:  2006        PMID： 17105946     DOI： 10.1196/annals.1369.048

Source DB:  PubMed          Journal:  Ann N Y Acad Sci        ISSN： 0077-8923            Impact factor:   5.691


  6 in total

1.  Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy.

Authors:  Lova Rakotomalala; Leo Studach; Wen-Horng Wang; Gerald Gregori; Ronald L Hullinger; Ourania Andrisani
Journal:  J Biol Chem       Date:  2008-08-08       Impact factor: 5.157

2.  Preliminary evaluation of a model of stimulant use, oxidative damage and executive dysfunction.

Authors:  Theresa Winhusen; Jessica Walker; Gregory Brigham; Daniel Lewis; Eugene Somoza; Jeff Theobald; Veronika Somoza
Journal:  Am J Drug Alcohol Abuse       Date:  2013-06-28       Impact factor: 3.829

Review 3.  Accumulation of nuclear DNA damage or neuron loss: molecular basis for a new approach to understanding selective neuronal vulnerability in neurodegenerative diseases.

Authors:  Ivona Brasnjevic; Patrick R Hof; Harry W M Steinbusch; Christoph Schmitz
Journal:  DNA Repair (Amst)       Date:  2008-05-23

4.  Genotoxicity of styrene-acrylonitrile trimer in brain, liver, and blood cells of weanling F344 rats.

Authors:  Cheryl A Hobbs; Rajendra S Chhabra; Leslie Recio; Michael Streicker; Kristine L Witt
Journal:  Environ Mol Mutagen       Date:  2012-02-20       Impact factor: 3.216

5.  Guanidinoacetate decreases antioxidant defenses and total protein sulfhydryl content in striatum of rats.

Authors:  Alexandra I Zugno; Francieli M Stefanello; Emilene B S Scherer; Cristiane Mattos; Carolina D Pederzolli; Vanessa M Andrade; Clovis M D Wannmacher; Moacir Wajner; Carlos S Dutra-Filho; Angela T S Wyse
Journal:  Neurochem Res       Date:  2008-03-15       Impact factor: 3.996

6.  Region-specific DNA alterations in focally induced seizures.

Authors:  Emanuela Cantafora; Filippo Sean Giorgi; Giada Frenzilli; Vittoria Scarcelli; Carla L Busceti; Marco Nigro; Margherita Bernardeschi; Francesco Fornai
Journal:  J Neural Transm (Vienna)       Date:  2014-04-20       Impact factor: 3.575
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.