Literature DB >> 1710339

Calpastatin and nucleotides stabilize cardiac calcium channel activity in excised patches.

C Romanin1, P Grösswagen, H Schindler.   

Abstract

The activity of single L-type Ca2+ channels is rapidly lost (run-down) when contact between the membrane and cytosol is interrupted. We have now achieved the stabilization of cardiac Ca2+ channel activity of guinea-pig ventricular myocytes by using either cytosol or defined components added to excised patches. The endogenous protease inhibitor, calpastatin, together with nucleotides, ATP + GTP, was found to prevent run-down as effectively as cardiac cytosolic solution. These results suggest the involvement of proteolysis by calpain in run-down of channel activity and enable the study of cardiac Ca2+ channel regulation with free access to both sides of the membrane.

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Year:  1991        PMID： 1710339     DOI： 10.1007/bf00370456

Source DB:  PubMed          Journal:  Pflugers Arch        ISSN： 0031-6768            Impact factor:   3.657


  36 in total

1.  Regulation of the cardiac calcium channel by protein phosphatases.

Authors:  J Hescheler; M Kameyama; W Trautwein; G Mieskes; H D Söling
Journal:  Eur J Biochem       Date:  1987-06-01

2.  Tissue extract recovers cardiac calcium channels from 'run-down'.

Authors:  M Kameyama; A Kameyama; T Nakayama; M Kaibara
Journal:  Pflugers Arch       Date:  1988-08       Impact factor: 3.657

3.  Phorbol ester increases calcium current and simulates the effects of angiotensin II on cultured neonatal rat heart myocytes.

Authors:  A Döşemeci; R S Dhallan; N M Cohen; W J Lederer; T B Rogers
Journal:  Circ Res       Date:  1988-02       Impact factor: 17.367

4.  Beta-adrenergic increase in the calcium conductance of cardiac myocytes studied with the patch clamp.

Authors:  G Brum; W Osterrieder; W Trautwein
Journal:  Pflugers Arch       Date:  1984-06       Impact factor: 3.657

5.  Modification of L-type calcium current by intracellularly applied trypsin in guinea-pig ventricular myocytes.

Authors:  J Hescheler; W Trautwein
Journal:  J Physiol       Date:  1988-10       Impact factor: 5.182

6.  Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

Authors:  O P Hamill; A Marty; E Neher; B Sakmann; F J Sigworth
Journal:  Pflugers Arch       Date:  1981-08       Impact factor: 3.657

7.  Reversible and irreversible activation of cyclic nucleotide phosphodiesterase: separation of the regulatory and catalytic domains by limited proteolysis.

Authors:  M H Krinks; J Haiech; A Rhoads; C B Klee
Journal:  Adv Cyclic Nucleotide Protein Phosphorylation Res       Date:  1984

8.  Calcium tolerant ventricular myocytes prepared by preincubation in a "KB medium".

Authors:  G Isenberg; U Klockner
Journal:  Pflugers Arch       Date:  1982-10       Impact factor: 3.657

9.  Effects of a protein phosphatase inhibitor, okadaic acid, on membrane currents of isolated guinea-pig cardiac myocytes.

Authors:  J Hescheler; G Mieskes; J C Rüegg; A Takai; W Trautwein
Journal:  Pflugers Arch       Date:  1988-08       Impact factor: 3.657

10.  Calcium channel selectivity for divalent and monovalent cations. Voltage and concentration dependence of single channel current in ventricular heart cells.

Authors:  P Hess; J B Lansman; R W Tsien
Journal:  J Gen Physiol       Date:  1986-09       Impact factor: 4.086
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  23 in total

1.  Modulation of the smooth-muscle L-type Ca2+ channel alpha1 subunit (alpha1C-b) by the beta2a subunit: a peptide which inhibits binding of beta to the I-II linker of alpha1 induces functional uncoupling.

Authors:  A Hohaus; M Poteser; C Romanin; N Klugbauer; F Hofmann; I Morano; H Haase; K Groschner
Journal:  Biochem J       Date:  2000-06-15       Impact factor: 3.857

2.  Molecular determinant for run-down of L-type Ca2+ channels localized in the carboxyl terminus of the 1C subunit.

Authors:  K J Kepplinger; G Förstner; H Kahr; K Leitner; P Pammer; K Groschner; N M Soldatov; C Romanin
Journal:  J Physiol       Date:  2000-11-15       Impact factor: 5.182

3.  Ion concentration-dependence of rat cardiac unitary L-type calcium channel conductance.

Authors:  A Guia; M D Stern; E G Lakatta; I R Josephson
Journal:  Biophys J       Date:  2001-06       Impact factor: 4.033

4.  Mechanosensitivity of N-type calcium channel currents.

Authors:  Barbara Calabrese; Iustin V Tabarean; Peter Juranka; Catherine E Morris
Journal:  Biophys J       Date:  2002-11       Impact factor: 4.033

5.  Phosphorylation restores activity of L-type calcium channels after rundown in inside-out patches from rabbit cardiac cells.

Authors:  K Ono; H A Fozzard
Journal:  J Physiol       Date:  1992-08       Impact factor: 5.182

6.  Regulation of muscle Cav1.1 channels by long-term depolarization involves proteolysis of the alpha1s subunit.

Authors:  E Carrillo; J M Galindo; M C García; J A Sánchez
Journal:  J Membr Biol       Date:  2004-06-01       Impact factor: 1.843

Review 7.  Regulation of L-type Ca2+ channels in the heart: overview of recent advances.

Authors:  Kaoru Yamaoka; Masaki Kameyama
Journal:  Mol Cell Biochem       Date:  2003-11       Impact factor: 3.396

8.  Two PEST-like motifs regulate Ca2+/calpain-mediated cleavage of the CaVbeta3 subunit and provide important determinants for neuronal Ca2+ channel activity.

Authors:  Alejandro Sandoval; Norma Oviedo; Abir Tadmouri; Traudy Avila; Michel De Waard; Ricardo Felix
Journal:  Eur J Neurosci       Date:  2006-05       Impact factor: 3.386

9.  A single amino acid mutation attenuates rundown of voltage-gated calcium channels.

Authors:  Xiao-Guang Zhen; Cheng Xie; Yoichi Yamada; Yun Zhang; Christina Doyle; Jian Yang
Journal:  FEBS Lett       Date:  2006-09-22       Impact factor: 4.124

10.  Mechanisms of antagonistic action of internal Ca2+ on serotonin-induced potentiation of Ca2+ currents in Helix neurones.

Authors:  P G Kostyuk; E A Lukyanetz
Journal:  Pflugers Arch       Date:  1993-06       Impact factor: 3.657
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