Literature DB >> 1710018

Inactivation of DNA polymerase by adenosine 2',3'-riboepoxide 5'-triphosphate allows estimation of the primers affinity.

V N Podust1, T O Korobeinicheva, G A Nevinsky, A S Levina, O I Lavrik.   

Abstract

Template-primer dependent inactivation of human DNA polymerase alpha and Klenow fragment of E. coli DNA polymerase I by adenosine 2',3'-riboepoxide 5'-triphosphate was used for quantitative analysis of the Kd values for oligonucleotide primers of different length. The Kd values are smaller by a factor of 2.5 than the Km values for the same primers determined in the reaction of DNA polymerization in the case of DNA polymerase alpha. The Kd and Km values are nearly the same for Klenow fragment. Such approach to the determination of Km/Kd ratio can likely be used for detailed quantitative analysis of DNA polymerases.

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Year:  1990        PMID： 1710018     DOI： 10.1007/bf00429892

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  12 in total

1.  Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase.

Authors:  R KITZ; I B WILSON
Journal:  J Biol Chem       Date:  1962-10       Impact factor: 5.157

2.  Apparent suicidal inactivation of DNA polymerase by adenosine 2',3'-riboepoxide 5'-triphosphate.

Authors:  M M Abboud; W J Sim; L A Loeb; A S Mildvan
Journal:  J Biol Chem       Date:  1978-05-25       Impact factor: 5.157

3.  Structure-function analysis of mononucleotides and short oligonucleotides in the priming of enzymatic DNA synthesis.

Authors:  G A Nevinsky; A G Veniaminova; A S Levina; V N Podust; O I Lavrik; E Holler
Journal:  Biochemistry       Date:  1990-02-06       Impact factor: 3.162

4.  [E. coli DNA polymerase. A study of the mechanism of primer binding using oligothymidylate analogs with ethylated internucleotide phosphate groups].

Authors:  A S Levina; G A Nevinskiĭ; O I Lavrik
Journal:  Bioorg Khim       Date:  1985-03

5.  Inactivation of DNA polymerase I (Klenow fragment) by adenosine 2',3'-epoxide 5'-triphosphate: evidence for the formation of a tight-binding inhibitor.

Authors:  C E Catalano; S J Benkovic
Journal:  Biochemistry       Date:  1989-05-16       Impact factor: 3.162

6.  [Template-primer-dependent inactivation of DNA polymerase alpha from human placenta by 2',3'-epoxyadenosine-5'-triphosphate].

Authors:  V N Podust; T O Korobeĭnicheva; G A Nevinskiĭ; V A Rikhter; T I Abramova; O I Lavrik
Journal:  Bioorg Khim       Date:  1990-02

7.  Protein-nucleic acid interaction in reactions catalyzed with DNA polymerases.

Authors:  D G Knorre; O I Lavrik; G A Nevinsky
Journal:  Biochimie       Date:  1988-05       Impact factor: 4.079

8.  Comparison of initiating abilities of primers of different length in polymerization reactions catalyzed by DNA polymerases from thermoacidophilic archaebacteria.

Authors:  I S Bukhrashvili; D Z Chinchaladze; O I Lavrik; A S Levina; G A Nevinsky; D A Prangishvili
Journal:  Biochim Biophys Acta       Date:  1989-06-01

9.  The efficiency of interaction of deoxyribonucleoside-5'-mono-, di- and triphosphates with the active centre of E. coli DNA polymerase I Klenow fragment.

Authors:  S V Doronin; G A Nevinsky; T O Malygina; V N Podust; V V Khomov; O I Lavrik
Journal:  FEBS Lett       Date:  1989-12-18       Impact factor: 4.124

10.  [DNA-polymerase alpha from human placenta. Effectiveness of interaction between oligothymidylates of different lengths and the template-binding site].

Authors:  G A Nevinskiĭ; V N Podust; A S Levina; O V Khalabuda; O I Lavrik
Journal:  Bioorg Khim       Date:  1986-03
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  1 in total

1.  Comparison of interactions of 5'-derivatives of deoxyoctathymidylate with human DNA polymerize alpha and HIV reverse transcriptase.

Authors:  S N Khodyreva; V N Podust; D S Sergeev; E M Ivanova; E I Frolova; A A Koshkin; T S Godovikova; V F Zarytova; M Ricchetti; O I Lavrik
Journal:  Mol Biol Rep       Date:  1993-06       Impact factor: 2.316
  1 in total

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