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Literature DB >> 1709694

Expression of processed core protein of hepatitis C virus in mammalian cells.

S Harada¹, Y Watanabe, K Takeuchi, T Suzuki, T Katayama, Y Takebe, I Saito, T Miyamura.

Abstract

A structural protein of hepatitis C virus (HCV) was expressed in monkey COS cells under the control of an exogenous promoter, and a protein of 22 kDa was identified by immunoblot analysis. This protein (p22), which was produced by processing in COS cells, reacted specifically to sera of chronic hepatitis C patients, and its coding region was mapped at the most amino-terminal part of the HCV polyprotein. These results suggested that the p22 protein is the nucleocapsid (core) protein of HCV. Moreover, the assay detecting antibody to p22 was found to be useful for early diagnosis of HCV infection.

Entities: Disease Species

Mesh：

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Year: 1991 PMID： 1709694 PMCID： PMC240954

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

20 in total

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9. Evidence for the participation of saccharide-lipids in the synthesis of the oligosaccharide chain of ovalbumin.

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32 in total

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5. Synthetic gene for the hepatitis C virus nucleocapsid protein.

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6. NS3 is a serine protease required for processing of hepatitis C virus polyprotein.

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7. Correlation of serum antibody titers against hepatitis C virus core protein with clinical features by western blot (immunoblot) analysis using a recombinant vaccinia virus expression system.

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