The direct chiral separations of fungicide enantiomers on amylopectin based chiral stationary phase by HPLC.

Amylopectin-tris(phenylcarbamate) was synthesized and coated to aminopropylsilica to prepare chiral stationary phase. The chiral separations of fungicide enantiomers were performed by the CSP using high-performance liquid chromatography. Mobile phase was n-hexane and isopropanol, and flow rate was 1.0 ml/min. Detection wavelength was 230 nm. The influence of the percentage of isopropanol in the mobile phase on the separations was studied. Twelve chiral fungicides were tested and seven of them were found to show stereoselectivity on the CSP. The enantiomers of metalaxyl and benalaxyl got near baseline separations and myclobutanil, hexconazole, tebuconazole, uniconazole, and paclobutrazol enantiomers were completely separated. The decreasing percentage of isopropanol in the mobile phase resulted in better separation and longer analysis time. The enantiomers were identified by a circular dichroism (CD) detector and the CD spectra of the individual enantiomers were also studied by online scanning. Copyright 2006 Wiley-Liss, Inc.