| Literature DB >> 17094943 |
Takashi Tsuyama1, Katsutoshi Inou, Masayuki Seki, Takahiko Seki, Yuji Kumata, Takayuki Kobayashi, Keiji Kimura, Fumio Hanaoka, Takemi Enomoto, Shusuke Tada.
Abstract
Smc6, a member of the structural maintenance of chromosomes (SMC) family of proteins, forms a complex with related Smc5. Genetic analyses of yeast have demonstrated the involvement of Smc6 in DNA repair and checkpoint responses. In this study, we investigated the role of the Smc5/6 complex in higher eukaryotes by analyzing its behavior in Xenopus laevis egg extracts. Smc5/6 was loaded onto chromatin during DNA replication in a manner dependent on the initiation of DNA synthesis, and it dissociated from chromatin during mitosis. Moreover, the induction of DNA double-strand breaks following replication did not significantly affect the amount of chromatin-associated Smc6. These findings suggest that the Smc5/6 complex is regulated during the cell cycle, presumably in anticipation of DNA damage that may arise during replication.Entities:
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Year: 2006 PMID： 17094943 DOI： 10.1016/j.bbrc.2006.10.133
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575