Literature DB >> 1709487

Antigenomic Hepatitis delta virus ribozymes self-cleave in 18 M formamide.

J B Smith1, G Dinter-Gottlieb.   

Abstract

The ribozymes derived from Hepatitis delta virus (HDV) RNA appear unique in their sequence requirements for self-cleavage. While truncating the 1679 nucleotide antigenomic HDV RNA, we have characterized the cleavage requirements of a number of ribozymes of intermediate length. Two of these, containing 186 and 106 HDV nucleotides respectively, cleaved to completion in the presence of 18 M formamide. The 186 nucleotide ribozyme also cleaved to completion in 10 M urea. Removal of an additional 10 nts from the 3' terminus of the 106 nt ribozyme resulted in a loss of the ability to cleave in high concentrations of the denaturants. The interaction of nucleotides near the cleavage site with a sequence within this 10 base region may confer unusual stability on these ribozymes.

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Year:  1991        PMID： 1709487      PMCID： PMC333855          DOI： 10.1093/nar/19.6.1285

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Self-cleavage of hepatitis delta virus genomic strand RNA is enhanced under partially denaturing conditions.

Authors:  S P Rosenstein; M D Been
Journal:  Biochemistry       Date:  1990-09-04       Impact factor: 3.162

2.  Structure and replication of the genome of the hepatitis delta virus.

Authors:  P J Chen; G Kalpana; J Goldberg; W Mason; B Werner; J Gerin; J Taylor
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

3.  Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sites.

Authors:  A C Forster; R H Symons
Journal:  Cell       Date:  1987-04-24       Impact factor: 41.582

4.  Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications.

Authors:  M Y Kuo; J Goldberg; L Coates; W Mason; J Gerin; J Taylor
Journal:  J Virol       Date:  1988-06       Impact factor: 5.103

5.  Sequence-specific endoribonuclease activity of the Tetrahymena ribozyme: enhanced cleavage of certain oligonucleotide substrates that form mismatched ribozyme-substrate complexes.

Authors:  A J Zaug; C A Grosshans; T R Cech
Journal:  Biochemistry       Date:  1988-12-13       Impact factor: 3.162

Review 6.  On finding all suboptimal foldings of an RNA molecule.

Authors:  M Zuker
Journal:  Science       Date:  1989-04-07       Impact factor: 47.728

7.  RNA catalytic properties of the minimum (-)sTRSV sequence.

Authors:  A Hampel; R Tritz
Journal:  Biochemistry       Date:  1989-06-13       Impact factor: 3.162

8.  Characterization of self-cleaving RNA sequences on the genome and antigenome of human hepatitis delta virus.

Authors:  M Y Kuo; L Sharmeen; G Dinter-Gottlieb; J Taylor
Journal:  J Virol       Date:  1988-12       Impact factor: 5.103

9.  Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site.

Authors:  A C Forster; R H Symons
Journal:  Cell       Date:  1987-07-03       Impact factor: 41.582

10.  RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.

Authors:  H N Wu; M M Lai
Journal:  Mol Cell Biol       Date:  1990-10       Impact factor: 4.272
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  11 in total

1.  Assaying synthetic ribozymes in plants: high-level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts.

Authors:  L Mazzolini; M Axelos; N Lescure; P Yot
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

2.  Random mutations to evaluate the role of bases at two important single-stranded regions of genomic HDV ribozyme.

Authors:  P K Kumar; Y A Suh; H Miyashiro; F Nishikawa; J Kawakami; K Taira; S Nishikawa
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

3.  Deletion of internal sequence on the HDV-ribozyme: elucidation of functionally important single-stranded loop regions.

Authors:  Y A Suh; P K Kumar; F Nishikawa; E Kayano; S Nakai; O Odai; S Uesugi; K Taira; S Nishikawa
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

Review 4.  Chemistry and Biology of Self-Cleaving Ribozymes.

Authors:  Randi M Jimenez; Julio A Polanco; Andrej Lupták
Journal:  Trends Biochem Sci       Date:  2015-10-15       Impact factor: 13.807

5.  Core-associated non-duplex sequences distinguishing the genomic and antigenomic self-cleaving RNAs of hepatitis delta virus.

Authors:  T S Wadkins; M D Been
Journal:  Nucleic Acids Res       Date:  1997-10-15       Impact factor: 16.971

6.  3-D models of the antigenomic ribozyme of the hepatitis delta agent with eight new contacts suggested by sequence analysis of 188 cDNA clones.

Authors:  A D Branch; J A Polaskova
Journal:  Nucleic Acids Res       Date:  1995-10-25       Impact factor: 16.971

7.  Inhibition of HIV-1 replication by ribozymes that show poor activity in vitro.

Authors:  P Crisell; S Thompson; W James
Journal:  Nucleic Acids Res       Date:  1993-11-11       Impact factor: 16.971

8.  A nested double pseudoknot is required for self-cleavage activity of both the genomic and antigenomic hepatitis delta virus ribozymes.

Authors:  T S Wadkins; A T Perrotta; A R Ferré-D'Amaré; J A Doudna; M D Been
Journal:  RNA       Date:  1999-06       Impact factor: 4.942

9.  Self-cleavage of a 71 nucleotide-long ribozyme derived from hepatitis delta virus genomic RNA.

Authors:  G Thill; M Blumenfeld; F Lescure; M Vasseur
Journal:  Nucleic Acids Res       Date:  1991-12-11       Impact factor: 16.971

10.  Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme.

Authors:  A T Perrotta; M D Been
Journal:  Nucleic Acids Res       Date:  1993-08-25       Impact factor: 16.971
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