BACKGROUND: Procyanidins are apple constituents with potential in colon cancer chemoprevention. MATERIALS AND METHODS: Human colon cancer derived metastatic cells (SW620), growing under standardized conditions, were exposed to procyanidins and lysosomotropic compounds. Growth, apoptosis and lysosomal integrity was determined using published methods. RESULTS: Lysosomotropic drugs (MDL 72527, phenylalanine methylester and chloroquine) amplified procyanidin-induced growth inhibition and apoptosis in SW620 cells at non-cytotoxic concentrations. The improved toxicity of the drug combinations relies primarily on the enhancement of lysosomal membrane permeability. CONCLUSION: Combinations with non-toxic concentrations of lysosomotropic compounds improve the anti-carcinogenic properties of apple procyanidins.
BACKGROUND:Procyanidins are apple constituents with potential in colon cancer chemoprevention. MATERIALS AND METHODS:Humancolon cancer derived metastatic cells (SW620), growing under standardized conditions, were exposed to procyanidins and lysosomotropic compounds. Growth, apoptosis and lysosomal integrity was determined using published methods. RESULTS: Lysosomotropic drugs (MDL 72527, phenylalanine methylester and chloroquine) amplified procyanidin-induced growth inhibition and apoptosis in SW620 cells at non-cytotoxic concentrations. The improved toxicity of the drug combinations relies primarily on the enhancement of lysosomal membrane permeability. CONCLUSION: Combinations with non-toxic concentrations of lysosomotropic compounds improve the anti-carcinogenic properties of appleprocyanidins.
Authors: Anastasia V Bagamanshina; Olga S Troitskaya; Anna A Nushtaeva; Anastasia Yu Yunusova; Marina O Starykovych; Elena V Kuligina; Yuri Ya Kit; Max Richter; Fabian Wohlfromm; Thilo Kähne; Inna N Lavrik; Vladimir A Richter; Olga A Koval Journal: Biomed Res Int Date: 2019-06-17 Impact factor: 3.411