BACKGROUND: Human papillomavirus (HPV) is the etiological agent of cervical cancer. HPV genotyping is important to determine the presence of high-risk types. Recently, a new HPV genotyping method, the Roche Linear array genotyping test, was introduced and is compared here with a sequencing-based HPV genotyping system. MATERIALS AND METHODS: A series of 102 women (age range 30-55 years) shown to be HPV DNA-positive by PCR were typed by sequencing and the Linear array genotyping assay. RESULTS: The sequence analysis revealed the presence of 80 single high-risk types and 22 single low-risk types. With the Linear array, single infections were found in 46 cases, double infections in 37 cases, triple infections in 12 cases, and more than three in 6 cases. One case positive by sequencing gave a negative result by Linear array. Altogether, a concordant single genotype was found in 93 (91.2%) out of the 102 cases and the single-type concordance between the two assays was significant (Spearman rho = 0.849, p = 0.0001; intraclass correlation coefficient (ICC) (ICC = 0.924, 95% CI 0.888-0.949) (p = 0.0001). The majority of the disparate results were due to the detection of multiple types by the Linear array. CONCLUSION: The Roche Linear array is a highly accurate assay for HPV genotyping. This is particularly true in the presence of multiple infections which DNA sequencing is unable to resolve.
BACKGROUND:Human papillomavirus (HPV) is the etiological agent of cervical cancer. HPV genotyping is important to determine the presence of high-risk types. Recently, a new HPV genotyping method, the Roche Linear array genotyping test, was introduced and is compared here with a sequencing-based HPV genotyping system. MATERIALS AND METHODS: A series of 102 women (age range 30-55 years) shown to be HPV DNA-positive by PCR were typed by sequencing and the Linear array genotyping assay. RESULTS: The sequence analysis revealed the presence of 80 single high-risk types and 22 single low-risk types. With the Linear array, single infections were found in 46 cases, double infections in 37 cases, triple infections in 12 cases, and more than three in 6 cases. One case positive by sequencing gave a negative result by Linear array. Altogether, a concordant single genotype was found in 93 (91.2%) out of the 102 cases and the single-type concordance between the two assays was significant (Spearman rho = 0.849, p = 0.0001; intraclass correlation coefficient (ICC) (ICC = 0.924, 95% CI 0.888-0.949) (p = 0.0001). The majority of the disparate results were due to the detection of multiple types by the Linear array. CONCLUSION: The Roche Linear array is a highly accurate assay for HPV genotyping. This is particularly true in the presence of multiple infections which DNA sequencing is unable to resolve.
Authors: Jill Koshiol; S Terence Dunn; Joan L Walker; Rosemary E Zuna; Mark Schiffman; Mark E Sherman; Michael A Gold; Richard A Allen; Roy Zhang; Sophia S Wang; Nicolas Wentzensen Journal: J Clin Microbiol Date: 2012-11-28 Impact factor: 5.948
Authors: Brenda Y Hernandez; Lynne R Wilkens; Xuemei Zhu; Pamela Thompson; Katharine McDuffie; Yurii B Shvetsov; Lori E Kamemoto; Jeffrey Killeen; Lily Ning; Marc T Goodman Journal: Emerg Infect Dis Date: 2008-06 Impact factor: 6.883
Authors: Mohamed A Youssef; Lobna Abdelsalam; Reem Abdelhameed Harfoush; Iman Mamdouh Talaat; Eman Elkattan; Abeer Mohey; Rana M A Abdella; Marwa Salah Farhan; Hany Ahmed Foad; Abeer Mostafa Elsayed; Naglaa A Elkinaai; Doaa Ghaith; Mohamed Elsayed Rashed; Mohamed Abd-El Ghafar; Yasser Khamis; Ahmed N Hosni Journal: Infect Agent Cancer Date: 2016-02-17 Impact factor: 2.965