Literature DB >> 17093309

Visible and fluorescent staining of two-dimensional gels.

François Chevalier1, Valérie Rofidal, Michel Rossignol.   

Abstract

Staining of two-dimensional gel constitutes a crucial step in comparative proteome analysis with respect to the number of proteins analyzed, the accuracy of spot quantification, and compatibility with mass spectrometry. The present chapter describes procedures for several visible and fluorescent dyes compatible with mass spectrometry: colloidal Coomassie blue, silver nitrate, Sypro Ruby, Deep Purple, and 5-hexadecanoylamino-fluorescein.

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Year:  2007        PMID： 17093309     DOI： 10.1385/1-59745-227-0:145

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Highlights on the capacities of "Gel-based" proteomics.

Authors:  François Chevalier
Journal:  Proteome Sci       Date:  2010-04-28       Impact factor: 2.480

Review 2.  A brief review of other notable protein detection methods on acrylamide gels.

Authors:  Biji T Kurien; R Hal Scofield
Journal:  Methods Mol Biol       Date:  2012

3.  A sulfhydryl-reactive ruthenium (II) complex and its conjugation to protein G as a universal reagent for fluorescent immunoassays.

Authors:  Jing-Tang Lin; Po-Chung Chen; Thirumani Venkatshwar Goud; Bor-Rong Huang; Tzu-Chau Lin; Jean-François Biellmann; Chien-Sheng Chen
Journal:  PLoS One       Date:  2012-04-26       Impact factor: 3.240

4.  Serum proteomic analysis reveals high frequency of haptoglobin deficiency and elevated thyroxine level in heroin addicts.

Authors:  Bing-Ying Zhou; Shi-Yan Yan; Wan-Lu Shi; Zhi Qu; Xin Zhao; Zhi-Min Liu; Xiao-Ping Pu
Journal:  PLoS One       Date:  2014-04-17       Impact factor: 3.240

Review 5.  Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis.

Authors:  François Chevalier
Journal:  Materials (Basel)       Date:  2010-10-20       Impact factor: 3.623
  5 in total

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