Literature DB >> 1709177

A simple assay for proteolysis of IGFBP-3.

G Lamson1, L C Giudice, R G Rosenfeld.   

Abstract

An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125IhIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37 degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

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Year:  1991        PMID： 1709177     DOI： 10.1210/jcem-72-6-1391

Source DB:  PubMed          Journal:  J Clin Endocrinol Metab        ISSN： 0021-972X            Impact factor:   5.958


  7 in total

Review 1.  Insulin-like growth factor (IGF) system in the bovine mammary gland and milk.

Authors:  C R Baumrucker; N E Erondu
Journal:  J Mammary Gland Biol Neoplasia       Date:  2000-01       Impact factor: 2.673

2.  Insulin-like growth factor I, IGF binding protein 3, and IGFBP protease activity: relation to anthropometric indices in solid tumours or leukaemia.

Authors:  B M Brennan; M Gill; L Pennells; O B Eden; A G Thomas; P E Clayton
Journal:  Arch Dis Child       Date:  1999-03       Impact factor: 3.791

3.  Glucose does not influence the insulin-like growth factor (JGF) binding to carrier proteins (IGFBPs): analysis of rat and human serum by western ligand blotting.

Authors:  S Cianfarani; V J Frost; M O Savage; J M Holly
Journal:  Experientia       Date:  1993-08-15

4.  Insulin-like growth factor binding protein-3 protease activity in the urine of children with chronic renal failure.

Authors:  D Y Lee; P Cohen; A M Krensky; R G Rosenfeld; P D Yorgin
Journal:  Pediatr Nephrol       Date:  1993-08       Impact factor: 3.714

5.  Insulin-like growth factor binding protein-3 and -5 are regulated by transforming growth factor-beta and retinoic acid in the human prostate adenocarcinoma cell line PC-3.

Authors:  V Hwa; Y Oh; R G Rosenfeld
Journal:  Endocrine       Date:  1997-06       Impact factor: 3.633

6.  Regulation of IGF binding proteins by FSH in human luteinizing granulosa cells.

Authors:  T Adachi; M Iwashita; A Kuroshima; Y Takeda
Journal:  J Assist Reprod Genet       Date:  1995-10       Impact factor: 3.412

7.  Microencapsulated octreotide pamoate in advanced gastrointestinal and pancreatic cancer: a phase I study.

Authors:  S I Helle; J Geisler; J P Poulsen; K Hestdal; K Meadows; W Collins; K M Tveit; J M Holly; P E Lønning
Journal:  Br J Cancer       Date:  1998-07       Impact factor: 7.640
  7 in total

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