Literature DB >> 17090918

Purification and properties of NADP-dependent shikimate dehydrogenase from Gluconobacter oxydans IFO 3244 and its application to enzymatic shikimate production.

Osao Adachi1, Yoshitaka Ano, Hirohide Toyama, Kazunobu Matsushita.   

Abstract

NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.

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Year:  2006        PMID： 17090918     DOI： 10.1271/bbb.60305

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  2 in total

1.  A mechanistic insight into hydrogen peroxide-mediated elicitation of bioactive xanthones in Hoppea fastigiata shoot cultures.

Authors:  Utkarsh Ravindra Moon; Adinpunya Mitra
Journal:  Planta       Date:  2016-04-08       Impact factor: 4.116

2.  The 5-Ketofructose Reductase of Gluconobacter sp. Strain CHM43 Is a Novel Class in the Shikimate Dehydrogenase Family.

Authors:  Thuy Minh Nguyen; Masaru Goto; Shohei Noda; Minenosuke Matsutani; Yuki Hodoya; Naoya Kataoka; Osao Adachi; Kazunobu Matsushita; Toshiharu Yakushi
Journal:  J Bacteriol       Date:  2021-09-08       Impact factor: 3.490
  2 in total

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