Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater.

A halophilic, Gram-negative, motile, non-sporulating bacterium designated strain FB1T was isolated from a wine-barrel-decalcification wastewater. The organism comprises straight rods and has a strictly respiratory metabolism with O2. Strain FB1T grows optimally at 20-30 degrees C and 5-6% NaCl. The predominant fatty acids were found to be C18:1omega9c (30.4%), C16:0 (25.7%), C12:0 3-OH (10.3%), C16:1omega9c (9.7%) and C16:1omega7c (8.4%). A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain forms a coherent cluster within the genus Marinobacter. The highest level of 16S rRNA gene sequence similarity (97.9%) exhibited by strain FB1T was with the type strain of Marinobacter excellens. However, the level of DNA-DNA relatedness between the novel strain and M. excellens CIP 107,686T was only 31.2%. The DNA G+C content of strain FB1T was 58.7 mol%. On the basis of phenotypic and genotypic characteristics, and also phylogenetic evidence, strain FB1T is considered to represent a novel species of the genus Marinobacter, for which the name Marinobacter vinifirmus sp. nov. is proposed. The type strain is FB1T (=DSM 17747T=CCUG 52119T).