Literature DB >> 17074502

A triaxial probe for on-line proteolysis coupled with hydrogen/deuterium exchange-electrospray mass spectrometry.

Maolian Chen1, Kelsey D Cook, Indu Kheterpal, Ronald Wetzel.   

Abstract

An on-line proteolysis system utilizing a triaxial electrospray probe was developed to aid localization of the hydrogen-bonding interaction sites in hydrogen/deuterium exchange-mass spectrometry (HDX-MS) studies of Abeta (1-40) fibrils. The probe allows delayed introduction of the organic solvent component needed for stable electrospray, thus enhancing hydrolysis performance relative to that of a coaxial probe. Effective on-line digestion was accomplished in approximately 12 s. The probe should be of general utility for HDX-MS studies of amyloid fibrils and other protein aggregates.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 17074502     DOI： 10.1016/j.jasms.2006.09.011

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  26 in total

1.  Enhanced prediction accuracy of protein secondary structure using hydrogen exchange Fourier transform infrared spectroscopy.

Authors:  B I Baello; P Pancoska; T A Keiderling
Journal:  Anal Biochem       Date:  2000-04-10       Impact factor: 3.365

2.  Hydrogen/deuterium exchange mass spectrometry analysis of protein aggregates.

Authors:  Indu Kheterpal; Kelsey D Cook; Ronald Wetzel
Journal:  Methods Enzymol       Date:  2006       Impact factor: 1.600

3.  Probing the non-covalent structure of proteins by amide hydrogen exchange and mass spectrometry.

Authors:  D L Smith; Y Deng; Z Zhang
Journal:  J Mass Spectrom       Date:  1997-02       Impact factor: 1.982

4.  Electrochemically induced pH changes resulting in protein unfolding in the ion source of an electrospray mass spectrometer.

Authors:  L Konermann; E A Silva; O F Sogbein
Journal:  Anal Chem       Date:  2001-10-15       Impact factor: 6.986

Review 5.  Investigation of protein folding by mass spectrometry.

Authors:  A Miranker; C V Robinson; S E Radford; C M Dobson
Journal:  FASEB J       Date:  1996-01       Impact factor: 5.191

6.  Hydrogen-deuterium (H/D) exchange mapping of Abeta 1-40 amyloid fibril secondary structure using nuclear magnetic resonance spectroscopy.

Authors:  Neil A Whittemore; Rajesh Mishra; Indu Kheterpal; Angela D Williams; Ronald Wetzel; Engin H Serpersu
Journal:  Biochemistry       Date:  2005-03-22       Impact factor: 3.162

7.  Site-specific amide hydrogen exchange in melittin probed by electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry.

Authors:  Hye Kyong Kweon; Kristina Håkansson
Journal:  Analyst       Date:  2005-10-14       Impact factor: 4.616

8.  Abeta amyloid fibrils possess a core structure highly resistant to hydrogen exchange.

Authors:  I Kheterpal; S Zhou; K D Cook; R Wetzel
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

9.  Enhanced correction methods for hydrogen exchange-mass spectrometric studies of amyloid fibrils.

Authors:  Indu Kheterpal; Ronald Wetzel; Kelsey D Cook
Journal:  Protein Sci       Date:  2003-03       Impact factor: 6.725

10.  Molecular modeling of the core of Abeta amyloid fibrils.

Authors:  Jun-tao Guo; Ronald Wetzel; Ying Xu
Journal:  Proteins       Date:  2004-11-01
View more
  3 in total

1.  Pressurized pepsin digestion in proteomics: an automatable alternative to trypsin for integrated top-down bottom-up proteomics.

Authors:  Daniel López-Ferrer; Konstantinos Petritis; Errol W Robinson; Kim K Hixson; Zhixin Tian; Jung Hwa Lee; Sang-Won Lee; Nikola Tolić; Karl K Weitz; Mikhail E Belov; Richard D Smith; Ljiljana Pasa-Tolić
Journal:  Mol Cell Proteomics       Date:  2010-07-12       Impact factor: 5.911

2.  Complementary peptide sequence coverage using alternative enzymes for on-line digestion with a triaxial electrospray probe.

Authors:  Andrew B Dykstra; Maolian Chen; Kelsey D Cook
Journal:  J Am Soc Mass Spectrom       Date:  2009-08-12       Impact factor: 3.109

3.  Development of an automated digestion and droplet deposition microfluidic chip for MALDI-TOF MS.

Authors:  Jeonghoon Lee; Harrison K Musyimi; Steven A Soper; Kermit K Murray
Journal:  J Am Soc Mass Spectrom       Date:  2008-04-08       Impact factor: 3.109
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.