| Literature DB >> 17072014 |
Cristina L Marolda1, Piya Lahiry, Enrique Vinés, Soledad Saldías, Miguel A Valvano.
Abstract
Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.Entities:
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Year: 2006 PMID： 17072014 DOI： 10.1385/1-59745-167-3:237
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745