Literature DB >> 17072008

Detailed structural analysis of N-glycans released from glycoproteins in SDS-PAGE gel bands using HPLC combined with exoglycosidase array digestions.

Louise Royle1, Catherine M Radcliffe, Raymond A Dwek, Pauline M Rudd.   

Abstract

In contrast to the linear sequences of protein and DNA, oligosaccharides are branched structures. In addition, almost all glycoproteins consist of a heterogeneous collection of differently glycosylated variants. Glycan analysis therefore requires high-resolution separation techniques that can provide detailed structural analysis, including both monosaccharide sequence and linkage information. This chapter describes how a combination of high-performance liquid chromatography (HPLC) and exoglycosidase enzyme array digestions can deliver quantitative glycan analysis of sugars released from glycoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel bands by matching HPLC elution positions with a database of standard glycans.

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Year:  2006        PMID： 17072008     DOI： 10.1385/1-59745-167-3:125

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  63 in total

1.  Circular trimers of gelatinase B/matrix metalloproteinase-9 constitute a distinct population of functional enzyme molecules differentially regulated by tissue inhibitor of metalloproteinases-1.

Authors:  Jennifer Vandooren; Benjamin Born; Inna Solomonov; Ewa Zajac; Radka Saldova; Michael Senske; Estefanía Ugarte-Berzal; Erik Martens; Philippe E Van den Steen; Jo Van Damme; Angeles Garcia-Pardo; Matheus Froeyen; Elena I Deryugina; James P Quigley; Søren K Moestrup; Pauline M Rudd; Irit Sagi; Ghislain Opdenakker
Journal:  Biochem J       Date:  2015-01-15       Impact factor: 3.857

2.  Mass Spectrometric Quantification of N-Linked Glycans by Reference to Exogenous Standards.

Authors:  Nickita Mehta; Mindy Porterfield; Weston B Struwe; Christian Heiss; Parastoo Azadi; Pauline M Rudd; Michael Tiemeyer; Kazuhiro Aoki
Journal:  J Proteome Res       Date:  2016-08-05       Impact factor: 4.466

3.  Groove-type recognition of chlamydiaceae-specific lipopolysaccharide antigen by a family of antibodies possessing an unusual variable heavy chain N-linked glycan.

Authors:  Omid Haji-Ghassemi; Sven Müller-Loennies; Radka Saldova; Mohankumar Muniyappa; Lore Brade; Pauline M Rudd; David J Harvey; Paul Kosma; Helmut Brade; Stephen V Evans
Journal:  J Biol Chem       Date:  2014-03-28       Impact factor: 5.157

4.  Effects of statins on the immunoglobulin G glycome.

Authors:  Toma Keser; Frano Vučković; Clara Barrios; Jonas Zierer; Annika Wahl; Akintunde O Akinkuolie; Jerko Štambuk; Natali Nakić; Tamara Pavić; Josipa Periša; Samia Mora; Christian Gieger; Cristina Menni; Tim D Spector; Olga Gornik; Gordan Lauc
Journal:  Biochim Biophys Acta Gen Subj       Date:  2017-03-02       Impact factor: 3.770

5.  Benchmark study of automatic annotation of MALDI-TOF N-glycan profiles.

Authors:  Alejandro E Brito; Doron Kletter; Mudita Singhal; Marshall Bern
Journal:  J Proteomics       Date:  2015-06-03       Impact factor: 4.044

6.  Type and branched pattern of N-glycans and their structural effect on the chicken egg allergen ovotransferrin: a comparison with ovomucoid.

Authors:  Hye Seong Hwang; Byung Sun Kim; Heajin Park; Ho-Young Park; Hee-Don Choi; Ha Hyung Kim
Journal:  Glycoconj J       Date:  2013-09-08       Impact factor: 2.916

Review 7.  Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses.

Authors:  L Renee Ruhaak; Gege Xu; Qiongyu Li; Elisha Goonatilleke; Carlito B Lebrilla
Journal:  Chem Rev       Date:  2018-03-19       Impact factor: 60.622

8.  Building a PGC-LC-MS N-glycan retention library and elution mapping resource.

Authors:  Jodie L Abrahams; Matthew P Campbell; Nicolle H Packer
Journal:  Glycoconj J       Date:  2017-09-13       Impact factor: 2.916

9.  Initiation of the alternative pathway of murine complement by immune complexes is dependent on N-glycans in IgG antibodies.

Authors:  Nirmal K Banda; Allyson K Wood; Kazue Takahashi; Brandt Levitt; Pauline M Rudd; Louise Royle; Jodie L Abrahams; Gregory L Stahl; V Michael Holers; William P Arend
Journal:  Arthritis Rheum       Date:  2008-10

Review 10.  Aberrant PSA glycosylation--a sweet predictor of prostate cancer.

Authors:  Sarah Gilgunn; Paul J Conroy; Radka Saldova; Pauline M Rudd; Richard J O'Kennedy
Journal:  Nat Rev Urol       Date:  2013-01-15       Impact factor: 14.432
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