BACKGROUND: Chimeric proteins may be used to direct cell-specific activity. Heparin-binding growth-associated molecule (HBGAM) binds to cell receptors that are relatively more robust on endothelial cells, and it may confer endothelial cell selectivity to potent angiogens such as fibroblast growth factor-1 (FGF-1). METHODS: By ligating fibroblast growth factor or its potent mutant, S130K, to HBGAM, we tested their effect on re-endothelialization after angioplasty injury by using a canine model. RESULTS: Both HBGAM/S130K- and HBGAM/FGF-1-treated arteries had increased neointimal mitotic index and re-endothelialization rates at 30 days compared with control arteries without inducing a significant increase in the neointimal thickness or the ratio of neointimal to medial thickness between treatment and control groups. CONCLUSION: HBGAM/S130K and HBGAM/FGF-1 facilitates endothelial healing without myointimal thickening after canine carotid artery balloon angioplasty injury. Application of these growth factors in fibrin glue may improve endothelialization clinically after angioplasty or endarterectomy.
BACKGROUND: Chimeric proteins may be used to direct cell-specific activity. Heparin-binding growth-associated molecule (HBGAM) binds to cell receptors that are relatively more robust on endothelial cells, and it may confer endothelial cell selectivity to potent angiogens such as fibroblast growth factor-1 (FGF-1). METHODS: By ligating fibroblast growth factor or its potent mutant, S130K, to HBGAM, we tested their effect on re-endothelialization after angioplasty injury by using a canine model. RESULTS: Both HBGAM/S130K- and HBGAM/FGF-1-treated arteries had increased neointimal mitotic index and re-endothelialization rates at 30 days compared with control arteries without inducing a significant increase in the neointimal thickness or the ratio of neointimal to medial thickness between treatment and control groups. CONCLUSION: HBGAM/S130K and HBGAM/FGF-1 facilitates endothelial healing without myointimal thickening after canine carotid artery balloon angioplasty injury. Application of these growth factors in fibrin glue may improve endothelialization clinically after angioplasty or endarterectomy.
Authors: Chan Woo Kim; Anastassia Pokutta-Paskaleva; Sandeep Kumar; Lucas H Timmins; Andrew D Morris; Dong-Won Kang; Sidd Dalal; Tatiana Chadid; Katie M Kuo; Julia Raykin; Haiyan Li; Hiromi Yanagisawa; Rudolph L Gleason; Hanjoong Jo; Luke P Brewster Journal: Circulation Date: 2017-08-04 Impact factor: 29.690
Authors: Bradley K Wacker; Shannon K Alford; Evan A Scott; Meghna Das Thakur; Gregory D Longmore; Donald L Elbert Journal: Biophys J Date: 2007-09-07 Impact factor: 4.033
Authors: Luke P Brewster; Cicely Washington; Eric M Brey; Andrew Gassman; Anu Subramanian; Jen Calceterra; William Wolf; Connie L Hall; William H Velander; Wilson H Burgess; Howard P Greisler Journal: Biomaterials Date: 2007-10-22 Impact factor: 12.479