| Literature DB >> 17070886 |
Keita Hoshino1, Haruhiko Isawa, Yoshio Tsuda, Kazuhiko Yano, Toshinori Sasaki, Masao Yuda, Tomohiko Takasaki, Mutsuo Kobayashi, Kyoko Sawabe.
Abstract
We found a new flavivirus that is widespread in Culex pipiens and other Culex mosquitoes in Japan. The virus isolate, named Culex flavivirus (CxFV), multiplied only in mosquito cell lines producing a moderate cytopathic effect, but did not grow in mammalian cells. The CxFV genome is single-stranded RNA, 10,834 nt in length and containing a single open reading frame encoding a polyprotein of 3362 aa with 5' and 3' untranslated regions (UTRs) of 91 and 657 nt, respectively. Phylogenetic analyses revealed that CxFV is closely related to the insect flaviviruses associated with Aedes mosquitoes, Cell fusing agent (CFA) and Kamiti River virus (KRV). The 3' UTR of CxFV contains four tandem repeats, which have sequence similarities to the two direct repeats in the CFA and KRV 3' UTRs. These results suggest that CxFV may be a new group of insect flaviviruses.Entities:
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Year: 2006 PMID： 17070886 DOI： 10.1016/j.virol.2006.09.039
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616