| Literature DB >> 17067580 |
Ammini Parvathi1, H Sanath Kumar, B Kenchanna Prakasha, Jieyuan Lu, Xuenian Xu, Wei Hu, Zheng Feng, Indrani Karunasagar, Iddya Karunasagar.
Abstract
Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C. sinensis. The primers designed from the nucleotide sequence data derived in this study were evaluated for their specificity and sensitivity for the detection of C. sinensis. The specific amplification products were obtained only with C. sinensis and no amplifications occurred with the DNA of closely related trematodes including Opisthorchis viverrini demonstrating the specificity of the assay. The novel PCR method described here will be useful for the quarantine of fishery products and evaluation of transmission status of clonorchiasis in the endemic areas.Entities:
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Year: 2006 PMID： 17067580 DOI： 10.1016/j.exppara.2006.09.010
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011