Literature DB >> 1706732

Identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rRNA restriction patterns.

T H Pennington1, C Harker, F Thomson-Carter.   

Abstract

A total of 1,417 staphylococcal and micrococcal strains were collected from the beards and scalps of 10 subjects over a period of 8 months. Sixteen strains identified as Staphylococcus epidermidis with an API system had distinctive yellow colonies on nutrient agar plates and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell polypeptide profiles similar to those of Staphylococcus capitis; this identification was confirmed by analysis of rRNA gene restriction patterns.

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Year:  1991        PMID： 1706732      PMCID： PMC269773          DOI： 10.1128/jcm.29.2.390-392.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Simplified scheme for routine identification of human Staphylococcus species.

Authors:  W E Kloos; K H Schleifer
Journal:  J Clin Microbiol       Date:  1975-01       Impact factor: 5.948

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns.

Authors:  F M Thomson-Carter; P E Carter; T H Pennington
Journal:  J Gen Microbiol       Date:  1989-07

4.  Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.

Authors:  F Grimont; P A Grimont
Journal:  Ann Inst Pasteur Microbiol       Date:  1986 Sep-Oct

5.  Staphylococcal whole-cell polypeptide analysis: evaluation as a taxonomic and typing tool.

Authors:  J Clink; T H Pennington
Journal:  J Med Microbiol       Date:  1987-02       Impact factor: 2.472

6.  Evaluation of a conventional routine method for identification of clinical isolates of coagulase-negative Staphylococcus and Micrococcus species. Comparison with API-Staph and API-Staph-Ident.

Authors:  B Gahrn-Hansen; O Heltberg; V T Rosdahl; P Søgaard
Journal:  Acta Pathol Microbiol Immunol Scand B       Date:  1987-10

7.  Identification of coagulase-negative staphylococci with the API staph system.

Authors:  C G Gemmell; J E Dawson
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

8.  Temporal study of staphylococcal species on the skin of human subjects in isolation and clonal analysis of Staphylococcus capitis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  A F Maggs; T H Pennington
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948
  8 in total
  5 in total

1.  Usefulness of the ID32 staph system and a method based on rRNA gene restriction site polymorphism analysis for species and subspecies identification of staphylococcal clinical isolates.

Authors:  O Chesneau; S Aubert; A Morvan; J L Guesdon; N el Solh
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Molecular characterization of Haemophilus ducreyi by ribosomal DNA fingerprinting.

Authors:  S K Sarafian; T C Woods; J S Knapp; B Swaminathan; S A Morse
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

3.  HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci.

Authors:  S H Goh; S Potter; J O Wood; S M Hemmingsen; R P Reynolds; A W Chow
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

4.  Genetic exchange of determinants for capsular polysaccharide biosynthesis between Klebsiella pneumoniae strains expressing serotypes K2 and K21a.

Authors:  I Ofek; K Kabha; A Athamna; G Frankel; D J Wozniak; D L Hasty; D E Ohman
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441

5.  Relationships among capsular structure, phagocytosis, and mouse virulence in Klebsiella pneumoniae.

Authors:  K Kabha; L Nissimov; A Athamna; Y Keisari; H Parolis; L A Parolis; R M Grue; J Schlepper-Schafer; A R Ezekowitz; D E Ohman
Journal:  Infect Immun       Date:  1995-03       Impact factor: 3.441
  5 in total

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