BACKGROUND AND OBJECTIVES: We evaluated if mRNA expression of survivin, an inhibitor of apoptosis, can be used to detect circulating tumor cells in peripheral blood of patients with various gastrointestinal cancers and if they decrease following complete surgical resection. METHODS: Blood samples from 40 gastrointestinal cancer patients were analyzed prior and following surgical resection by direct quantitative real-time reverse transcriptase-PCR (RT-PCR) assays. RESULTS: Survivin mRNA expression was pre-operatively detected in 35 of 40 cancer patients (88%). Post-operative survivin levels were significantly lower than pre-operative levels in 59% of resected patients and were non-detectable in 38% (Wilcoxon rank test: P < 0.04). CONCLUSIONS: This is the first report showing that direct quantitative real-time RT-PCR analysis of survivin mRNA expression in peripheral blood of patients with gastrointestinal cancers is technically feasible. Survivin mRNA levels fall significantly following complete resection and might become a molecular marker for the completeness of surgical resection.
BACKGROUND AND OBJECTIVES: We evaluated if mRNA expression of survivin, an inhibitor of apoptosis, can be used to detect circulating tumor cells in peripheral blood of patients with various gastrointestinal cancers and if they decrease following complete surgical resection. METHODS: Blood samples from 40 gastrointestinal cancerpatients were analyzed prior and following surgical resection by direct quantitative real-time reverse transcriptase-PCR (RT-PCR) assays. RESULTS: Survivin mRNA expression was pre-operatively detected in 35 of 40 cancerpatients (88%). Post-operative survivin levels were significantly lower than pre-operative levels in 59% of resected patients and were non-detectable in 38% (Wilcoxon rank test: P < 0.04). CONCLUSIONS: This is the first report showing that direct quantitative real-time RT-PCR analysis of survivin mRNA expression in peripheral blood of patients with gastrointestinal cancers is technically feasible. Survivin mRNA levels fall significantly following complete resection and might become a molecular marker for the completeness of surgical resection.
Authors: Jan Brabender; Daniel Vallböhmer; Peter Grimminger; Andreas C Hoffmann; Frederike Ling; Georg Lurje; Elfriede Bollschweiler; Paul M Schneider; Arnulf H Hölscher; Ralf Metzger Journal: J Gastrointest Surg Date: 2008-09-03 Impact factor: 3.452
Authors: Andreas-Claudius Hoffmann; Daniel Vallböhmer; Klaus Prenzel; Ralf Metzger; Michaela Heitmann; Susanne Neiss; Fredericke Ling; Arnulf H Hölscher; Paul M Schneider; Jan Brabender Journal: J Cancer Res Clin Oncol Date: 2009-03-04 Impact factor: 4.553
Authors: Barry M Dent; Laura F Ogle; Rachel L O'Donnell; Nicholas Hayes; Ujjal Malik; Nicola J Curtin; Alan V Boddy; E Ruth Plummer; Richard J Edmondson; Helen L Reeves; Felicity E B May; David Jamieson Journal: Int J Cancer Date: 2015-08-19 Impact factor: 7.396