Zi-jiang Chen1, Yuan Li, Jing-mei Hu, Mei Li. 1. Reproductive Medicine Center, Shandong University and Shandong Provincial Hospital, Jinan 250021, China.
Abstract
OBJECTIVE: To assess the feasibility and clinical value of cryopreservation of human oocytes. METHODS: Human oocytes were collected during stimulated cycle and then vitrificated with cryoloop. Surviving oocytes were thawed and then inseminated by intracytoplasmic sperm injection (ICSI). The fertilized oocytes were further cultured in vitro for 48 - 72 hours. High-quality embryos were transferred to the patients of infertility with informed consent. RESULTS: A total of 235 oocytes were vitrificated and thawed. The survival and fertilization rates of the oocytes were 71.9% and 72.5% respectively. Forty-one embryos were transferred to 11 patients. Two of the 11 patients achieved pregnancy, one of which gave birth of 2 healthy female twin babies and the other was at the 16 th week of pregnancy. The mother and babies were all healthy. CONCLUSION: Human oocyte vitrification is safe and feasible.
OBJECTIVE: To assess the feasibility and clinical value of cryopreservation of human oocytes. METHODS:Human oocytes were collected during stimulated cycle and then vitrificated with cryoloop. Surviving oocytes were thawed and then inseminated by intracytoplasmic sperm injection (ICSI). The fertilized oocytes were further cultured in vitro for 48 - 72 hours. High-quality embryos were transferred to the patients of infertility with informed consent. RESULTS: A total of 235 oocytes were vitrificated and thawed. The survival and fertilization rates of the oocytes were 71.9% and 72.5% respectively. Forty-one embryos were transferred to 11 patients. Two of the 11 patients achieved pregnancy, one of which gave birth of 2 healthy female twin babies and the other was at the 16 th week of pregnancy. The mother and babies were all healthy. CONCLUSION:Human oocyte vitrification is safe and feasible.
Authors: Eun Kyung Ryu; Yong Soo Hur; Ji Young Ann; Ja Young Maeng; Miji Park; Jeong Hyun Park; Jung Yoon; San Hyun Yoon; Chang Young Hur; Won Don Lee; Jin Ho Lim Journal: Clin Exp Reprod Med Date: 2012-12-31