Literature DB >> 17063504

Evaluation of a novel vesicular stomatitis virus pseudotype-based assay for detection of neutralizing antibody responses to SARS-CoV.

Shuetsu Fukushi1, Tetsuya Mizutani, Masayuki Saijo, Ichiro Kurane, Fumihiro Taguchi, Masato Tashiro, Shigeru Morikawa.   

Abstract

Severe acute respiratory syndrome (SARS)-coronavirus (SARS-CoV) is the causative agent of SARS. The S protein of SARS-CoV is a major target for neutralizing antibodies (Nabs) in infected patients. We developed a neutralization assay using a recombinant vesicular stomatitis virus (VSV) bearing SARS-CoV-S protein (VSV-SARS-St19). A total of 56 serum samples collected from 22 healthcare workers in the Hanoi French Hospital during the SARS epidemic in 2003 were evaluated and compared to the conventional neutralizing assay using infectious SARS-CoV. The results of the neutralization assay using VSV-SARS-St19 pseudotype showed good correlations with those using infectious SARS-CoV. The newly developed neutralization assay was more sensitive to low antibody titers in serum samples. Thus, the VSV-SARS-St19 is a useful tool for detecting Nabs against SARS-CoV.

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Year:  2006        PMID： 17063504     DOI： 10.1002/jmv.20732

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  17 in total

1.  Development of a poliovirus neutralization test with poliovirus pseudovirus for measurement of neutralizing antibody titer in human serum.

Authors:  Minetaro Arita; Masae Iwai; Takaji Wakita; Hiroyuki Shimizu
Journal:  Clin Vaccine Immunol       Date:  2011-08-31

2.  A seroepidemiologic study of Reston ebolavirus in swine in the Philippines.

Authors:  Yusuke Sayama; Catalino Demetria; Mariko Saito; Rachel R Azul; Satoshi Taniguchi; Shuetsu Fukushi; Tomoki Yoshikawa; Itoe Iizuka; Tetsuya Mizutani; Ichiro Kurane; Fidelino F Malbas; Socorro Lupisan; Davinio P Catbagan; Samuel B Animas; Rieldrin G Morales; Emelinda L Lopez; Karen Rose C Dazo; Magdalena S Cruz; Remigio Olveda; Masayuki Saijo; Hitoshi Oshitani; Shigeru Morikawa
Journal:  BMC Vet Res       Date:  2012-06-18       Impact factor: 2.741

3.  Development and Applications of VSV Vectors Based on Cell Tropism.

Authors:  Hideki Tani; Shigeru Morikawa; Yoshiharu Matsuura
Journal:  Front Microbiol       Date:  2012-01-18       Impact factor: 5.640

Review 4.  Serological assays based on recombinant viral proteins for the diagnosis of arenavirus hemorrhagic fevers.

Authors:  Shuetsu Fukushi; Hideki Tani; Tomoki Yoshikawa; Masayuki Saijo; Shigeru Morikawa
Journal:  Viruses       Date:  2012-10-12       Impact factor: 5.048

5.  A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition.

Authors:  M Eugenia Dieterle; Denise Haslwanter; Robert H Bortz; Ariel S Wirchnianski; Gorka Lasso; Olivia Vergnolle; Shawn A Abbasi; J Maximilian Fels; Ethan Laudermilch; Catalina Florez; Amanda Mengotto; Duncan Kimmel; Ryan J Malonis; George Georgiev; Jose Quiroz; Jason Barnhill; Liise-Anne Pirofski; Johanna P Daily; John M Dye; Jonathan R Lai; Andrew S Herbert; Kartik Chandran; Rohit K Jangra
Journal:  Cell Host Microbe       Date:  2020-07-03       Impact factor: 21.023

6.  Development of a recombinant replication-deficient rabies virus-based bivalent-vaccine against MERS-CoV and rabies virus and its humoral immunogenicity in mice.

Authors:  Hirofumi Kato; Mutsuyo Takayama-Ito; Itoe Iizuka-Shiota; Shuetsu Fukushi; Guillermo Posadas-Herrera; Madoka Horiya; Masaaki Satoh; Tomoki Yoshikawa; Souichi Yamada; Shizuko Harada; Hikaru Fujii; Miho Shibamura; Takuya Inagaki; Kinjiro Morimoto; Masayuki Saijo; Chang-Kweng Lim
Journal:  PLoS One       Date:  2019-10-07       Impact factor: 3.240

7.  Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus.

Authors:  C M Chan; Herman Tse; S S Y Wong; P C Y Woo; S K P Lau; L Chen; B J Zheng; J D Huang; K Y Yuen
Journal:  J Clin Virol       Date:  2009-04-01       Impact factor: 3.168

8.  A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein.

Authors:  Yoshihiro Kaku; Akira Noguchi; Glenn A Marsh; Jennifer A McEachern; Akiko Okutani; Kozue Hotta; Boldbaatar Bazartseren; Shuetsu Fukushi; Christopher C Broder; Akio Yamada; Satoshi Inoue; Lin-Fa Wang
Journal:  J Virol Methods       Date:  2009-05-09       Impact factor: 2.014

9.  Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA.

Authors:  Shuetsu Fukushi; Aiko Fukuma; Takeshi Kurosu; Shumpei Watanabe; Masayuki Shimojima; Kazuya Shirato; Naoko Iwata-Yoshikawa; Noriyo Nagata; Kazuo Ohnishi; Manabu Ato; Simenew Keskes Melaku; Hiroshi Sentsui; Masayuki Saijo
Journal:  J Virol Methods       Date:  2017-10-06       Impact factor: 2.014

Review 10.  COVID-19, an emerging coronavirus infection: advances and prospects in designing and developing vaccines, immunotherapeutics, and therapeutics.

Authors:  Kuldeep Dhama; Khan Sharun; Ruchi Tiwari; Maryam Dadar; Yashpal Singh Malik; Karam Pal Singh; Wanpen Chaicumpa
Journal:  Hum Vaccin Immunother       Date:  2020-03-18       Impact factor: 3.452
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