| Literature DB >> 1706309 |
R Anand1, D J Ogilvie, R Butler, J H Riley, R S Finniear, S J Powell, J C Smith, A F Markham.
Abstract
The gene responsible for cystic fibrosis (CF) has recently been identified. Coding sequence for the cystic fibrosis transmembrane conductance regulator (CFTR) spans at least 230 kb of the human genome. Although all 27 exons of the gene are represented in cosmid or bacteriophage clones, there are still several gaps in the physical map of this region. It should be possible to complete the map and to clone the entire CFTR gene in a single fragment of DNA using a yeast artificial chromosome (YAC) vector. Herein we describe the construction and physical mapping of a 1.5-Mb YAC contig which encompasses D7S8 (J3.11) and D7S23 (KM19), two genetic loci flanking the CF locus. One of the clones in the contig, 37AB12, contains a 310-kb YAC which includes the entire CFTR gene and flanking sequence in both the 5' and 3' directions.Entities:
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Year: 1991 PMID： 1706309 DOI： 10.1016/0888-7543(91)90229-8
Source DB: PubMed Journal: Genomics ISSN： 0888-7543 Impact factor: 5.736