Literature DB >> 170624

Concerning the subcellular distribution of 3beta-hydroxysteroid dehydrogenase/isomerase in the rat adrenal.

A M Moustafa, S B Koritz.   

Abstract

The distribution of 3beta-hydroxysteroid dehydrogenase/isomerase in the subcellular fractions of rat adrenal gland has been determined. Based on the total activity found, 55% was in the microsomal fraction, 10% in the heavy-mitochondrial fraction, 3% in the light-mitochondrial fraction and 26% in the fraction consisting of cell-debris plus nuclei. Ninety-five percent of the total activity was recovered in the fractions. Approximately half the activity in the heavy-mitochondrial fraction could be accounted for by microsomal contamination.

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Year:  1975        PMID： 170624     DOI： 10.3181/00379727-149-38906

Source DB:  PubMed          Journal:  Proc Soc Exp Biol Med        ISSN： 0037-9727


  6 in total

1.  Light and electron microscopic immunocytochemistry on the localization of 3 beta-hydroxysteroid dehydrogenase/isomerase in the bovine adrenal cortical cells.

Authors:  K Ishimura; T Yoshinaga-Hirabayashi; H Fujita; H Ishii-Ohba; H Inano; B Tamaoki
Journal:  Histochemistry       Date:  1988

2.  A quantitative cytochemical method for the demonstration of delta5,3beta-hydroxysteroid dehydrogenase activity in unfixed tissue sections of rat ovary.

Authors:  W R Robertson
Journal:  Histochemistry       Date:  1979-02-21

3.  Ultracytochemical demonstration and probable localization of 3beta-hydroxysteroid dehydrogenase activity with a ferricyanide technique.

Authors:  J P Berchtold
Journal:  Histochemistry       Date:  1977-01-24

4.  The ultrastructural localization of the enzymes related to steroid hormone metabolism in the guinea-pig testis.

Authors:  G Bara
Journal:  Histochem J       Date:  1979-01

5.  In situ demonstration of the activity of 3 beta-hydroxysteroid dehydrogenase on steroid hormone secreting cells within the paraaortic lymph nodes of golden hamster.

Authors:  K Tohya; R Sugata; S Toda; M Kimura
Journal:  Experientia       Date:  1989-08-15

6.  Mitochondrial 3 beta-hydroxysteroid dehydrogenase (HSD) is essential for the synthesis of progesterone by corpora lutea: an hypothesis.

Authors:  John C Chapman; Jose R Polanco; Soohong Min; Sandra D Michael
Journal:  Reprod Biol Endocrinol       Date:  2005-04-03       Impact factor: 5.211
  6 in total

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