OBJECTIVE: Radiation has been shown to enhance intercellular communication in the skin and lungs through an increase of connexin43 (Cx43) expression. If analogous Cx43 up-regulation is induced in the diseased heart, it would provide a new perspective in radiation therapy for arrhythmias. The aim of the present study is to test this hypothesis. METHODS: Non-transmural myocardial infarction (MI) was created in 24 rabbits by microsphere injection into the coronary arteries. Twenty-four rabbits without MI were used as controls. Targeted external heavy ion beam irradiation (THIR; 15 Gy) was applied 2 weeks after MI with an accelerator (HIMAC, Chiba, Japan). RESULTS: The THIR was associated with an increase of Cx43 mRNA and protein levels in the left ventricle in control as well as in MI rabbits. THIR also increased lateralization of Cx43, which was no longer colocalized with cadherins. In MI hearts, immunoreactive Cx43 signals were reduced in the peri-infarct zone, and the reduction was reversed by THIR. In-vivo epicardial potential mapping on the free wall (64 unipolar electrodes to cover 7 x 7 mm) in MI hearts revealed reduced conduction velocity, whereas dispersion of the activation-recovery interval (ARI) was increased compared with controls, and these changes were reversed by THIR. The vulnerability for ventricular tachyarrhythmias (VT/VF), which was estimated by programmed stimulation, was increased in MI hearts, and this increased vulnerability to arrhythmias was reversed by THIR. CONCLUSIONS: THIR increases Cx43 expression, improves the conductivity, decreases the spatial heterogeneity of repolarization, and reduces the vulnerability of rabbit hearts to ventricular arrhythmias after MI. THIR could have an antiarrhythmic potential through an improvement of electrical coupling.
OBJECTIVE: Radiation has been shown to enhance intercellular communication in the skin and lungs through an increase of connexin43 (Cx43) expression. If analogous Cx43 up-regulation is induced in the diseased heart, it would provide a new perspective in radiation therapy for arrhythmias. The aim of the present study is to test this hypothesis. METHODS: Non-transmural myocardial infarction (MI) was created in 24 rabbits by microsphere injection into the coronary arteries. Twenty-four rabbits without MI were used as controls. Targeted external heavy ion beam irradiation (THIR; 15 Gy) was applied 2 weeks after MI with an accelerator (HIMAC, Chiba, Japan). RESULTS: The THIR was associated with an increase of Cx43 mRNA and protein levels in the left ventricle in control as well as in MI rabbits. THIR also increased lateralization of Cx43, which was no longer colocalized with cadherins. In MI hearts, immunoreactive Cx43 signals were reduced in the peri-infarct zone, and the reduction was reversed by THIR. In-vivo epicardial potential mapping on the free wall (64 unipolar electrodes to cover 7 x 7 mm) in MI hearts revealed reduced conduction velocity, whereas dispersion of the activation-recovery interval (ARI) was increased compared with controls, and these changes were reversed by THIR. The vulnerability for ventricular tachyarrhythmias (VT/VF), which was estimated by programmed stimulation, was increased in MI hearts, and this increased vulnerability to arrhythmias was reversed by THIR. CONCLUSIONS:THIR increases Cx43 expression, improves the conductivity, decreases the spatial heterogeneity of repolarization, and reduces the vulnerability of rabbit hearts to ventricular arrhythmias after MI. THIR could have an antiarrhythmic potential through an improvement of electrical coupling.
Authors: Martijn H van der Ree; Edith M T Dieleman; Jorrit Visser; Judit A Adam; Rianne H A de Bruin-Bon; Rianne M A J de Jong; Wiert F Hoeksema; Arend Mosterd; Jippe C Balt; R Nils Planken; Brian V Balgobind; Pieter G Postema Journal: Adv Radiat Oncol Date: 2022-05-20
Authors: H Immo Lehmann; Christian Graeff; Palma Simoniello; Anna Constantinescu; Mitsuru Takami; Patrick Lugenbiel; Daniel Richter; Anna Eichhorn; Matthias Prall; Robert Kaderka; Fine Fiedler; Stephan Helmbrecht; Claudia Fournier; Nadine Erbeldinger; Ann-Kathrin Rahm; Rasmus Rivinius; Dierk Thomas; Hugo A Katus; Susan B Johnson; Kay D Parker; Jürgen Debus; Samuel J Asirvatham; Christoph Bert; Marco Durante; Douglas L Packer Journal: Sci Rep Date: 2016-12-20 Impact factor: 4.379
Authors: Felicitas Rapp; Palma Simoniello; Julia Wiedemann; Karola Bahrami; Valeria Grünebaum; Svetlana Ktitareva; Marco Durante; P Lugenbiel; D Thomas; H Immo Lehmann; Douglas L Packer; Christian Graeff; Claudia Fournier Journal: Sci Rep Date: 2019-03-21 Impact factor: 4.379