OBJECTIVE: To identify a new circulating recombinant form (CRF) of HIV-1 comprising two circulating subtypes in the southern region in Brazil, subtypes B and C. METHODS: A total of 152 HIV-positive patients followed at two hospitals in southern Brazil had their viral pol genes isolated by reverse transcriptase-polymerase chain reaction (PCR) from plasma. PCR products were sequenced and phylogenetically analysed using HIV-1 subtype reference sequences. Six full-length subtype C viruses from Brazil previously described as 'pure' strains were included in the analysis. Sequences suggestive of recombination were analysed by boot scanning and phylogenetic analyses of separate fragments. The common ancestry of recombinant strains was evaluated by similarity plot and informative site analyses. RESULTS: : HIV-1 subtypes commonly found in Brazil (B, C and F1) were observed. Sixty-two viruses were initially assigned as subtype C, but 15 viruses clustered in a separate internal clade. Pol from two full-length genomes of subtype C viruses grouped together with those samples. Boot scanning analysis showed that all 17 viruses had the same recombinant structure, with a 240 base pair fragment of subtype B in the middle of the reverse transcriptase pol region. Subtype B assignment of this fragment was confirmed by phylogenetic analyses using different methods of tree inference and cluster robustness tests. Mosaics were shown to have a common ancestry. CONCLUSION: As CRF_BC represents 11% of the HIV-1 viruses circulating in the southern region of the country, which borders several south American countries, the assessment of its spread is of pivotal importance to the HIV/AIDS epidemic in Brazil and Latin America.
OBJECTIVE: To identify a new circulating recombinant form (CRF) of HIV-1 comprising two circulating subtypes in the southern region in Brazil, subtypes B and C. METHODS: A total of 152 HIV-positive patients followed at two hospitals in southern Brazil had their viral pol genes isolated by reverse transcriptase-polymerase chain reaction (PCR) from plasma. PCR products were sequenced and phylogenetically analysed using HIV-1 subtype reference sequences. Six full-length subtype C viruses from Brazil previously described as 'pure' strains were included in the analysis. Sequences suggestive of recombination were analysed by boot scanning and phylogenetic analyses of separate fragments. The common ancestry of recombinant strains was evaluated by similarity plot and informative site analyses. RESULTS: : HIV-1 subtypes commonly found in Brazil (B, C and F1) were observed. Sixty-two viruses were initially assigned as subtype C, but 15 viruses clustered in a separate internal clade. Pol from two full-length genomes of subtype C viruses grouped together with those samples. Boot scanning analysis showed that all 17 viruses had the same recombinant structure, with a 240 base pair fragment of subtype B in the middle of the reverse transcriptase pol region. Subtype B assignment of this fragment was confirmed by phylogenetic analyses using different methods of tree inference and cluster robustness tests. Mosaics were shown to have a common ancestry. CONCLUSION: As CRF_BC represents 11% of the HIV-1 viruses circulating in the southern region of the country, which borders several south American countries, the assessment of its spread is of pivotal importance to the HIV/AIDS epidemic in Brazil and Latin America.
Authors: Mario P S Chin; Sook-Kyung Lee; Jianbo Chen; Olga A Nikolaitchik; Douglas A Powell; Mathew J Fivash; Wei-Shau Hu Journal: J Mol Biol Date: 2008-02-09 Impact factor: 5.469
Authors: Ludimila Paula Vaz Cardoso; Alexsander Augusto da Silveira; Roberta Barbosa Lopes Francisco; Mônica Nogueira da Guarda Reis; Mariane Martins de Araújo Stefani Journal: AIDS Res Hum Retroviruses Date: 2011-07-06 Impact factor: 2.205
Authors: Alexsander Augusto da Silveira; Ludimila Paula Vaz Cardoso; Roberta Barbosa Lopes Francisco; Mariane Martins de Araújo Stefani Journal: AIDS Res Hum Retroviruses Date: 2011-07-26 Impact factor: 2.205
Authors: Ana T Dumans; Cláudia C Barreto; André F Santos; Mônica Arruda; Thatiana M Sousa; Elizabeth S Machado; Ester C Sabino; Rodrigo M Brindeiro; Amílcar Tanuri; Alberto J Duarte; Marcelo A Soares Journal: Infect Genet Evol Date: 2008-10-17 Impact factor: 3.342
Authors: Sabri Saeed Sanabani; Evelyn Regina de Souza Pastena; Walter Kleine Neto; Claudia C Barreto; Kelly T Ferrari; Erika M N Kalmar; Suzete Ferreira; Ester Cerdeira Sabino Journal: Virol J Date: 2009-06-16 Impact factor: 4.099
Authors: Sabri Saeed Sanabani; Rodrigo Pessôa; Ana Carolina Soares de Oliveira; Vanessa Pouza Martinez; Maria Teresa Maidana Giret; Regina Célia de Menezes Succi; Karina Carvalho; Claudia Satiko Tomiyama; Douglas F Nixon; Ester Cerdeira Sabino; Esper Georges Kallas Journal: PLoS One Date: 2013-05-07 Impact factor: 3.240