Literature DB >> 1705263

Monoclonal immunofluorescence compared with silver stain for investigating Pneumocystis carinii pneumonia.

J Midgley1, P A Parsons, D C Shanson, O A Husain, N Francis.   

Abstract

Two hundred and eighty two specimens from 220 patients positive for HIV with respiratory tract symptoms, or febrile illness, or both, were examined for the presence of Pneumocystis carinii. Specimens were either induced sputum samples or bronchoalveolar lavage fluids. To establish the optimal method for laboratory diagnosis a comparison was made of detection of the organism by use of monoclonal antibody and immunofluorescence with conventional silver staining methods. Three commercially available reagents for immunofluorescence were also compared. Immunofluorescence was significantly more sensitive than the silver stain and the best results for immunofluorescence were obtained using. Northumbria Biologicals Ltd reagents.

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Year:  1991        PMID： 1705263      PMCID： PMC497021          DOI： 10.1136/jcp.44.1.75

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  7 in total

1.  Increased sensitivity of immunofluorescence for detection of Pneumocystis carinii.

Authors:  J Midgley; P Parsons; T R Leigh; J V Collins; D C Shanson; O A Husain; J N Harcourt-Webster
Journal:  Lancet       Date:  1989 Dec 23-30       Impact factor: 79.321

2.  Diagnosis of Pneumocystis carinii pneumonia by non-experts.

Authors:  E Dournon; P Rajagopalan; F Albert; F Camus; C Marche; J McLauchlin; D Samuel; A G Taylor
Journal:  Lancet       Date:  1989-01-14       Impact factor: 79.321

3.  Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum with use of monoclonal antibodies.

Authors:  J A Kovacs; V L Ng; H Masur; G Leoung; W K Hadley; G Evans; H C Lane; F P Ognibene; J Shelhamer; J E Parrillo
Journal:  N Engl J Med       Date:  1988-03-10       Impact factor: 91.245

4.  Detection of Pneumocystis carinii by fluorescent-antibody stain using a combination of three monoclonal antibodies.

Authors:  V J Gill; G Evans; F Stock; J E Parrillo; H Masur; J A Kovacs
Journal:  J Clin Microbiol       Date:  1987-10       Impact factor: 5.948

5.  Sputum induction for diagnosis of Pneumocystis carinii pneumonia.

Authors:  T R Leigh; P Parsons; C Hume; O A Husain; B Gazzard; J V Collins
Journal:  Lancet       Date:  1989-07-22       Impact factor: 79.321

6.  Monoclonal antibodies to Pneumocystis carinii: identification of specific antigens and characterization of antigenic differences between rat and human isolates.

Authors:  J A Kovacs; J L Halpern; B Lundgren; J C Swan; J E Parrillo; H Masur
Journal:  J Infect Dis       Date:  1989-01       Impact factor: 5.226

7.  Prospective evaluation of a monoclonal antibody in diagnosis of Pneumocystis carinii pneumonia.

Authors:  J A Kovacs; V Gill; J C Swan; F Ognibene; J Shelhamer; J E Parrillo; H Masur
Journal:  Lancet       Date:  1986-07-05       Impact factor: 79.321
  7 in total
  3 in total

1.  Application and staining patterns of commercial anti-Pneumocystis carinii monoclonal antibodies.

Authors:  K Elvin; E Linder
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

Review 2.  Diagnosing Pneumocystis jirovecii pneumonia: A review of current methods and novel approaches.

Authors:  Marjorie Bateman; Rita Oladele; Jay K Kolls
Journal:  Med Mycol       Date:  2020-11-10       Impact factor: 4.076

Review 3.  Update on special techniques in routine cytopathology.

Authors:  I D Buley
Journal:  J Clin Pathol       Date:  1993-10       Impact factor: 3.411
  3 in total

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