BACKGROUND: Accumulating evidence has demonstrated that the NT2 embryonal carcinoma cell line and multipotential stem cells found in BM, mesenchymal stromal cells (MSC), have the ability to differentiate into a wide variety of cell types. This study was designed to explore the efficacy of these two human stem cell types as a graft source for the treatment of demyelinating disorders such as Krabbe's disease and multiple sclerosis (MS). METHODS: We examined the engraftment and in vivo differentiation of adult MSC and NT2 cells after transplantation into two demyelinating environments, the neonatal and postnatal twitcher mouse brain. RESULTS: Both types of xenografts led to anatomical integration, without tumor formation, and remained viable in the normal and twitcher mouse brain, showing differentiation into neurons, astrocytes and oligodendrocytes. DISCUSSION: This study represents a platform for further stem cell transplantation studies in the twitcher model and potentially has important therapeutic implications.
BACKGROUND: Accumulating evidence has demonstrated that the NT2 embryonal carcinoma cell line and multipotential stem cells found in BM, mesenchymal stromal cells (MSC), have the ability to differentiate into a wide variety of cell types. This study was designed to explore the efficacy of these two human stem cell types as a graft source for the treatment of demyelinating disorders such as Krabbe's disease and multiple sclerosis (MS). METHODS: We examined the engraftment and in vivo differentiation of adult MSC and NT2 cells after transplantation into two demyelinating environments, the neonatal and postnatal twitcher mouse brain. RESULTS: Both types of xenografts led to anatomical integration, without tumor formation, and remained viable in the normal and twitcher mouse brain, showing differentiation into neurons, astrocytes and oligodendrocytes. DISCUSSION: This study represents a platform for further stem cell transplantation studies in the twitcher model and potentially has important therapeutic implications.
Authors: Cynthia B Ripoll; Mette Flaat; Jessica Klopf-Eiermann; Jeanne M Fisher-Perkins; Cynthia B Trygg; Brittni A Scruggs; Marjorie L McCants; Helen Paige Leonard; Amy F Lin; Shijia Zhang; Michelle E Eagle; Xavier Alvarez; Yu Teh Li; Su Chen Li; Jeffrey M Gimble; Bruce A Bunnell Journal: Stem Cells Date: 2011-01 Impact factor: 6.277
Authors: Shawna E Wicks; Heaven Londot; Bo Zhang; Jennifer Dowden; Jessica Klopf-Eiermann; Jeanne M Fisher-Perkins; Cynthia B Trygg; Brittni A Scruggs; Xiujuan Zhang; Jeffrey M Gimble; Bruce A Bunnell; Paul J Pistell Journal: Behav Brain Res Date: 2011-08-05 Impact factor: 3.332
Authors: Stefan Haubenwallner; Matthias Katschnig; Ulrike Fasching; Silke Patz; Christa Trattnig; Natascha Andraschek; Gerda Grünbacher; Markus Absenger; Stephan Laske; Clemens Holzer; Werner Balika; Manuela Wagner; Ute Schäfer Journal: J Mater Sci Mater Med Date: 2014-02-28 Impact factor: 3.896
Authors: Brittni A Scruggs; Xiujuan Zhang; Annie C Bowles; Peter A Gold; Julie A Semon; Jeanne M Fisher-Perkins; Shijia Zhang; Ryan W Bonvillain; Leann Myers; Su Chen Li; Allan V Kalueff; Bruce A Bunnell Journal: Stem Cells Date: 2013-08 Impact factor: 6.277
Authors: Kimberly A Terrell; Terri A Rasmussen; Cyndi Trygg; Bruce A Bunnell; Wayne R Buck Journal: J Neurosci Methods Date: 2007-02-25 Impact factor: 2.390
Authors: Brittni A Scruggs; Annie C Bowles; Xiujuan Zhang; Julie A Semon; Evan J Kyzar; Leann Myers; Allan V Kalueff; Bruce A Bunnell Journal: Behav Brain Res Date: 2012-08-20 Impact factor: 3.332