| Literature DB >> 17048840 |
Yoshinaga Yoshimura1, Daisuke Okamura, Masayuki Ogino, Kenzo Fujimoto.
Abstract
We describe a highly efficient template-directed photoligation of oligodeoxynucleotides (ODNs) through 5-carbamoylvinyl-2'-deoxycytidine ((CV)C). When an ODN containing (CV)C at the 5' end was photoirradiated with an ODN containing a pyrimidine base at the 3' end in the presence of template DNA, efficient photoligation was observed without any byproduct formation. Single nucleotide differences can be successfully distinguished by using photoligation-based DNA chip assay. [structure: see text]Entities:
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Year: 2006 PMID： 17048840 DOI： 10.1021/ol0619462
Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005