OBJECTIVE: To establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine. METHOD: A Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm. RESULT: Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively. CONCLUSION: This method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.
OBJECTIVE: To establish an HPLC method for the determination of three kind of diesterditerpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine. METHOD: A Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm. RESULT: Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively. CONCLUSION: This method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.