Literature DB >> 17046541

Alteration of substrate specificity of fructosyl-amino acid oxidase from Ulocladium sp. JS-103.

Maki Fujiwara1, Jun-ichi Sumitani, Shinji Koga, Issei Yoshioka, Takuji Kouzuma, Shigeyuki Imamura, Takashi Kawaguchi, Motoo Arai.   

Abstract

We showed by random mutagenesis that one-amino-acid substitution at Arg94 in fructosyl-amino acid oxidase from Ulocladium sp. JS-103 enhanced substrate specificity toward fructosyl valine (FV), a model compound of hemoglobin A(1c). Kinetic analysis showed that the specificity of the R94W mutant enzyme toward FV was 14-fold that of the wild-type enzyme. The mutant enzyme obtained will be useful in developing an enzymatic measurement method for hemoglobin A(1c).

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Year:  2006        PMID： 17046541     DOI： 10.1263/jbb.102.241

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  5 in total

Review 1.  Review of fructosyl amino acid oxidase engineering research: a glimpse into the future of hemoglobin A1c biosensing.

Authors:  Stefano Ferri; Seungsu Kim; Wakako Tsugawa; Koji Sode
Journal:  J Diabetes Sci Technol       Date:  2009-05-01

2.  Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum.

Authors:  Weiqiong Gan; Feng Gao; Keke Xing; Minze Jia; Haiping Liu; Weimin Gong
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-03-20       Impact factor: 1.056

3.  Engineering fructosyl peptide oxidase to improve activity toward the fructosyl hexapeptide standard for HbA1c measurement.

Authors:  Stefano Ferri; Yusuke Miyamoto; Akane Sakaguchi-Mikami; Wakako Tsugawa; Koji Sode
Journal:  Mol Biotechnol       Date:  2013-07       Impact factor: 2.695

4.  Crystal structure of the deglycating enzyme fructosamine oxidase (amadoriase II).

Authors:  François Collard; Jianye Zhang; Ina Nemet; Kaustubha R Qanungo; Vincent M Monnier; Vivien C Yee
Journal:  J Biol Chem       Date:  2008-07-30       Impact factor: 5.157

5.  Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis.

Authors:  Noriyuki Ogawa; Takehide Kimura; Fumi Umehara; Yuki Katayama; Go Nagai; Keiko Suzuki; Kazuo Aisaka; Yukie Maruyama; Takafumi Itoh; Wataru Hashimoto; Kousaku Murata; Michio Ichimura
Journal:  Sci Rep       Date:  2019-01-30       Impact factor: 4.379
  5 in total

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