Literature DB >> 17045265

In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin Va.

Xufeng Wu1, Take Sakamoto, Fang Zhang, James R Sellers, John A Hammer.   

Abstract

Rab27a and melanophilin (Mlph) are required in vivo to form a melanosome receptor for myosin Va in which Rab27a anchored in the melanosome membrane recruits Mlph, which in turn recruits myosin Va. Here, we show by reconstitution using purified proteins that Rab27a and Mlph are sufficient to form a transport complex with myosin Va in vitro. These results suggest that additional proteins are not required in vivo for assembly of the myosin Va receptor, although other proteins may associate with this tripartite complex to regulate its activity and/or to assist Rab27a in anchoring the complex to the melanosome membrane.

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Year:  2006        PMID： 17045265     DOI： 10.1016/j.febslet.2006.09.047

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  27 in total

Review 1.  Principles of unconventional myosin function and targeting.

Authors:  M Amanda Hartman; Dina Finan; Sivaraj Sivaramakrishnan; James A Spudich
Journal:  Annu Rev Cell Dev Biol       Date:  2011-05-31       Impact factor: 13.827

Review 2.  Walking to work: roles for class V myosins as cargo transporters.

Authors:  John A Hammer; James R Sellers
Journal:  Nat Rev Mol Cell Biol       Date:  2011-12-07       Impact factor: 94.444

3.  Slip sliding away with myosin V.

Authors:  John A Hammer; Xufeng Wu
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-20       Impact factor: 11.205

Review 4.  Regulation of class V myosin.

Authors:  Ning Zhang; Lin-Lin Yao; Xiang-Dong Li
Journal:  Cell Mol Life Sci       Date:  2017-07-20       Impact factor: 9.261

5.  Update on the regulation of mammalian melanocyte function and skin pigmentation.

Authors:  Taisuke Kondo; Vincent J Hearing
Journal:  Expert Rev Dermatol       Date:  2011-02-01

Review 6.  Use of fluorescent techniques to study the in vitro movement of myosins.

Authors:  Christopher Toepfer; James R Sellers
Journal:  Exp Suppl       Date:  2014

7.  Myosin-Va mediates RNA distribution in primary fibroblasts from multiple organs.

Authors:  Verônica P Salerno; Aldo Calliari; D William Provance; José R Sotelo-Silveira; José R Sotelo; John A Mercer
Journal:  Cell Motil Cytoskeleton       Date:  2008-05

8.  Direct observation of the uncapping of capping protein-capped actin filaments by CARMIL homology domain 3.

Authors:  Ikuko Fujiwara; Kirsten Remmert; John A Hammer
Journal:  J Biol Chem       Date:  2009-11-19       Impact factor: 5.157

9.  Myosin IIA associates with NK cell lytic granules to enable their interaction with F-actin and function at the immunological synapse.

Authors:  Keri B Sanborn; Gregory D Rak; Saumya Y Maru; Korey Demers; Analisa Difeo; John A Martignetti; Michael R Betts; Rémi Favier; Pinaki P Banerjee; Jordan S Orange
Journal:  J Immunol       Date:  2009-06-01       Impact factor: 5.422

Review 10.  Myosin V from head to tail.

Authors:  K M Trybus
Journal:  Cell Mol Life Sci       Date:  2008-05       Impact factor: 9.261
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