| Literature DB >> 17041743 |
A Hohner1, J Bayer, J O Rädler.
Abstract
The phase behavior of DOPE/DOTAP-DNA complexes in phase-separated oil(dodecane)/water mixtures was explored using Small Angle X-Ray Scattering (SAXS) and Fluorescence Correlation Spectroscopy (FCS). Inverse micelles of DNA with cationic-lipid coating were found in the oil phase. Varying the ratio between cationic and neutral lipids a transition from wormlike to spherical structures is observed for both long ( approximately 75000bp) and short (30-1246bp) DNA. In contrast to lipid/DNA complexes in the water phase, there is no indication of condensed liquid-crystalline structures in the non-polar phase. In fact, FCS measurements on short DNA oligomers complexed with cationic lipid in alkane give clear evidence for monomeric inverse micelles of DNA. Dilution series revealed a critical lower concentration of lipids and DNA for observing lipid/DNA micelles.Entities:
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Year: 2006 PMID： 17041743 DOI： 10.1140/epje/i2006-10043-y
Source DB: PubMed Journal: Eur Phys J E Soft Matter ISSN： 1292-8941 Impact factor: 1.890