AIMS AND BACKGROUND: Phyllodes tumor of the breast with osteosarcomatous differentiation is rare and very little is known about its molecular profile. METHODS AND STUDY DESIGN: An immunohistochemical panel with 37 primary antibodies including cytokeratins, mesenchymal markers, key regulators of the cell cycle, oncogenes, apoptosis-related proteins, metalloproteinases and their inhibitors was performed on a formalin-fixed paraffin-embedded sample of phyllodes tumor with osteosarcomatous differentiation in a 49-year-old woman. RESULTS: Antiapoptotic stimuli (survivin) predominated in sarcomatous cells. Antiproteolytic stimuli (TIMP-1, TIMP-2 and PAI) were preponderant in all cells, a surprising fact in view of the aggressiveness of the neoplasm. The immunoprofile of the osteoblastic and stromal cells was quite similar, except for c-erbB-3, c-myc, cyclin D1 and p21. Both exhibited positive cells for actin, MyoD1 and GFAP. CONCLUSIONS: Our results suggest that this osteosarcoma may have originated from metaplasia of stromal cells that underwent a malignant change.
AIMS AND BACKGROUND: Phyllodes tumor of the breast with osteosarcomatous differentiation is rare and very little is known about its molecular profile. METHODS AND STUDY DESIGN: An immunohistochemical panel with 37 primary antibodies including cytokeratins, mesenchymal markers, key regulators of the cell cycle, oncogenes, apoptosis-related proteins, metalloproteinases and their inhibitors was performed on a formalin-fixed paraffin-embedded sample of phyllodes tumor with osteosarcomatous differentiation in a 49-year-old woman. RESULTS: Antiapoptotic stimuli (survivin) predominated in sarcomatous cells. Antiproteolytic stimuli (TIMP-1, TIMP-2 and PAI) were preponderant in all cells, a surprising fact in view of the aggressiveness of the neoplasm. The immunoprofile of the osteoblastic and stromal cells was quite similar, except for c-erbB-3, c-myc, cyclin D1 and p21. Both exhibited positive cells for actin, MyoD1 and GFAP. CONCLUSIONS: Our results suggest that this osteosarcoma may have originated from metaplasia of stromal cells that underwent a malignant change.
Authors: Guerino Barbalaco Neto; Claudia Rossetti; Natalia A Souza; Fernando LA Fonseca; Ligia Ajaime Azzalis; Virginia Berlanga Campos Junqueira; Vitor E Valenti; Luiz Carlos de Abreu Journal: Eur J Med Res Date: 2012-04-25 Impact factor: 2.175
Authors: Nurul Atiah Mohd Ali; Ahmad Fazlin Nasaruddin; Syarah Syamimi Mohamed; Wan Faiziah Wan Rahman Journal: Asian Pac J Cancer Prev Date: 2020-09-01