Literature DB >> 17035494

Feasibility of freeze-dried sera for serological and molecular biological detection of hepatitis B and C viruses.

Waka Ohishi1, Saeko Fujiwara, Gen Suzuki, Takeshi Kishi, Misae Sora, Shinsuke Matsuura, Masayuki Hakoda, Yoshimi Tatsukawa, Michiko Yamada, Kazuaki Chayama.   

Abstract

We compared hepatitis B virus (HBV) surface antigen, anti-hepatitis C virus (HCV) antibody, and HCV RNA quantification in frozen and freeze-dried serum samples to assess the usefulness of freeze-dried sera for detection of HBV and HCV. The results indicated that freeze-dried sera as well as frozen sera can be useful for serological and molecular biological analyses of HBV and HCV.

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Year:  2006        PMID： 17035494      PMCID： PMC1698376          DOI： 10.1128/JCM.00621-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Simple and reliable method for detection and genotyping of hepatitis C virus RNA in dried blood spots stored at room temperature.

Authors:  Mariacarmela Solmone; Enrico Girardi; Francesco Costa; Leopoldo Pucillo; Giuseppe Ippolito; Maria R Capobianchi
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

2.  Prevalence of anti-hepatitis C virus antibody and chronic liver disease among atomic bomb survivors.

Authors:  S Fujiwara; S Kusumi; J Cologne; M Akahoshi; K Kodama; H Yoshizawa
Journal:  Radiat Res       Date:  2000-07       Impact factor: 2.841

3.  Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature.

Authors:  K Abe; N Konomi
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

4.  Dried sera for confirming blood-borne virus infections (HCV, HTLV-I, HIV & HBsAg).

Authors:  P C Das; A H de Vries; R L McShine; C T Sibinga
Journal:  Transfus Med       Date:  1996-12       Impact factor: 2.019

5.  Maternal antibody to hepatitis B core antigen detected in dried neonatal blood spot samples.

Authors:  D M Tappin; K Greer; S Cameron; R Kennedy; A J Brown; R W Girdwood
Journal:  Epidemiol Infect       Date:  1998-10       Impact factor: 2.451

6.  Usefulness of dried blood samples for quantification and molecular characterization of HBV-DNA.

Authors:  Rosendo Jardi; Francisco Rodriguez-Frias; Maria Buti; Melanie Schaper; Auristela Valdes; Mariano Martinez; Rafael Esteban; Jaime Guardia
Journal:  Hepatology       Date:  2004-07       Impact factor: 17.425

7.  Prevalence of hepatitis B virus infection among atomic bomb survivors.

Authors:  Saeko Fujiwara; Gerald B Sharp; John B Cologne; Shizuyo Kusumi; Masazumi Akahoshi; Kazunori Kodama; Gen Suzuki; Hiroshi Yoshizawa
Journal:  Radiat Res       Date:  2003-06       Impact factor: 2.841
  7 in total

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