Literature DB >> 17031431

A high-throughput screening assay for hydroxynitrile lyase activity.

Jennifer Andexer1, Jan-Karl Guterl, Martina Pohl, Thorsten Eggert.   

Abstract

A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 17031431     DOI： 10.1039/b607863j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Evolution of a Catalytic Mechanism.

Authors:  Alissa Rauwerdink; Mark Lunzer; Titu Devamani; Bryan Jones; Joanna Mooney; Zhi-Jun Zhang; Jian-He Xu; Romas J Kazlauskas; Antony M Dean
Journal:  Mol Biol Evol       Date:  2015-12-16       Impact factor: 16.240

2.  Uncovering divergent evolution of α/β-hydrolases: a surprising residue substitution needed to convert Hevea brasiliensis hydroxynitrile lyase into an esterase.

Authors:  David M Nedrud; Hui Lin; Gilsinia Lopez; Santosh K Padhi; Graig A Legatt; Romas J Kaz-Lauskas
Journal:  Chem Sci       Date:  2014-11       Impact factor: 9.825

3.  Functional prokaryotic RubisCO from an oceanic metagenomic library.

Authors:  Brian Witte; David John; Boris Wawrik; John H Paul; David Dayan; F Robert Tabita
Journal:  Appl Environ Microbiol       Date:  2010-03-12       Impact factor: 4.792

4.  Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism.

Authors:  Bryan J Jones; Zsófia Bata; Romas J Kazlauskas
Journal:  ACS Catal       Date:  2017-05-15       Impact factor: 13.084

5.  Hydroxynitrile lyase defends Arabidopsis against Tetranychus urticae.

Authors:  Ana Arnaiz; M Estrella Santamaria; Irene Rosa-Diaz; Irene Garcia; Sameer Dixit; Saul Vallejos; Cecilia Gotor; Manuel Martinez; Vojislava Grbic; Isabel Diaz
Journal:  Plant Physiol       Date:  2022-08-01       Impact factor: 8.005

6.  Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases.

Authors:  Titu Devamani; Alissa M Rauwerdink; Mark Lunzer; Bryan J Jones; Joanna L Mooney; Maxilmilien Alaric O Tan; Zhi-Jun Zhang; Jian-He Xu; Antony M Dean; Romas J Kazlauskas
Journal:  J Am Chem Soc       Date:  2016-01-15       Impact factor: 15.419

Review 7.  Interplay of metagenomics and in vitro compartmentalization.

Authors:  Manuel Ferrer; Ana Beloqui; José María Vieites; María Eugenia Guazzaroni; Ilana Berger; Amir Aharoni
Journal:  Microb Biotechnol       Date:  2008-10-13       Impact factor: 5.813
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.