| Literature DB >> 17030905 |
Takako Osaki, Tomoko Hanawa, Taki Manzoku, Minoru Fukuda, Hayato Kawakami, Hidekazu Suzuki1, Hiroyuki Yamaguchi2, Xu Yan3, Haruhiko Taguchi, Satoshi Kurata, Shigeru Kamiya.
Abstract
Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (AI-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0.3 % agar medium, motility of H. pylori HPKY08 (luxS : : cat) was significantly lower than that of wild-type H. pylori TK1402. The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/AI-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS(-) mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.Entities:
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Year: 2006 PMID： 17030905 DOI： 10.1099/jmm.0.46660-0
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472