Literature DB >> 17030158

Protein A chromatography for antibody purification.

Sophia Hober1, Karin Nord, Martin Linhult.   

Abstract

Staphylococcal protein A (SPA) is one of the first discovered immunoglobulin binding molecules and has been extensively studied during the past decades. Due to its affinity to immunoglobulins, SPA has found widespread use as a tool in the detection and purification of antibodies and the molecule has been further developed to one of the most employed affinity purification systems. Interestingly, a minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications. This review will focus on the development of different affinity molecules and matrices for usage in antibody purification.

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Year:  2006        PMID： 17030158     DOI： 10.1016/j.jchromb.2006.09.030

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  52 in total

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3.  Optical tweezers to study single protein A/immunoglobulin G interactions at varying conditions.

Authors:  Mathias Salomo; Ulrich F Keyser; Marc Struhalla; Friedrich Kremer
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4.  Electrophilic affibodies forming covalent bonds to protein targets.

Authors:  Lotta Holm; Paul Moody; Mark Howarth
Journal:  J Biol Chem       Date:  2009-09-15       Impact factor: 5.157

5.  Examination of glycan profiles from IgG-depleted human immunoglobulins facilitated by microscale affinity chromatography.

Authors:  Martin Svoboda; Benjamin F Mann; John A Goetz; Milos V Novotny
Journal:  Anal Chem       Date:  2012-03-13       Impact factor: 6.986

6.  Cloning and Expression Analysis of Human Amelogenin in Nicotiana benthamiana Plants by Means of a Transient Expression System.

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7.  Immunoglobulin G structure and rheumatoid factor epitopes.

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Journal:  PLoS One       Date:  2019-06-14       Impact factor: 3.240

8.  Production and characterisation of a neutralising chimeric antibody against botulinum neurotoxin A.

Authors:  Julie Prigent; Christelle Mazuet; Didier Boquet; Patricia Lamourette; Hervé Volland; Michel R Popoff; Christophe Créminon; Stéphanie Simon
Journal:  PLoS One       Date:  2010-10-08       Impact factor: 3.240

9.  Exploring the feasibility of selective depletion of T lymphocyte subsets by whole blood immunoadsorption cytapheresis.

Authors:  M Belak; C R Valeri; D G Wright
Journal:  Clin Exp Immunol       Date:  2007-10-09       Impact factor: 4.330

10.  Isolation and characterization of chimeric human Fc-expressing proteins using protein a membrane adsorbers and a streamlined workflow.

Authors:  Monica M Burdick; Nathan M Reynolds; Eric W Martin; Jacquelyn V Hawes; Grady E Carlson; Chaz M Cuckler; Michael C Bates; Steven R Barthel; Charles J Dimitroff
Journal:  J Vis Exp       Date:  2014-01-08       Impact factor: 1.355
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