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Literature DB >> 17028219

Limited diagnostic capacities of two commercial assays for the detection of Leptospira immunoglobulin M antibodies in Laos.

Stuart D Blacksell¹, Lee Smythe, Rattanaphone Phetsouvanh, Michael Dohnt, Rudy Hartskeerl, Meegan Symonds, Andrew Slack, Manivanh Vongsouvath, Viengmone Davong, Olay Lattana, Simmaly Phongmany, Valy Keolouangkot, Nicholas J White, Nicholas P J Day, Paul N Newton.

Abstract

The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira "gold standard" microscopic agglutination test.

Mesh：

Substances：
Immunoglobulin M

Year: 2006 PMID： 17028219 PMCID： PMC1595324 DOI： 10.1128/CVI.00219-06

Source DB: PubMed Journal: Clin Vaccine Immunol ISSN： 1556-679X

11 in total

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Authors: Simaly Phongmany; Jean-Marc Rolain; Rattanaphone Phetsouvanh; Stuart D Blacksell; Vimone Soukkhaseum; Bouachanh Rasachack; Khamphong Phiasakha; Surn Soukkhaseum; Khamthavi Frichithavong; Vang Chu; Valy Keolouangkhot; Bertrand Martinez-Aussel; Ko Chang; Chirapha Darasavath; Oudayvone Rattanavong; Siho Sisouphone; Mayfong Mayxay; Sisouphane Vidamaly; Philippe Parola; Changpeng Thammavong; Mayboun Heuangvongsy; Bounkong Syhavong; Didier Raoult; Nicholas J White; Paul N Newton
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25 in total

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Journal: Malays J Med Sci Date: 2012-07

4. An Improved Enzyme-Linked Immunoassay for the Detection of Leptospira-Specific Antibodies.

Authors: Hua-Wei Chen; Heather Lukas; Kira Becker; Giulia Weissenberger; Eric S Halsey; Carolina Guevara; Enrique Canal; Eric Hall; Ryan C Maves; Drake H Tilley; Lynn Kuo; Tadeusz J Kochel; Wei-Mei Ching
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Authors: Daša Podgoršek; Tjaša Cerar; Mateja Logar; Gorazd Lešničar; Tatjana Remec; Zvonko Baklan; Emil Pal; Eva Ružić-Sabljić
Journal: Wien Klin Wochenschr Date: 2015-09-17 Impact factor: 1.704

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Authors: Xu'ai Lin; Yin Chen; Jie Yan
Journal: Clin Vaccine Immunol Date: 2008-09-30

8. Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.

Authors: Hua-Wei Chen; Zhiwen Zhang; Eric S Halsey; Carolina Guevara; Enrique Canal; Eric Hall; Ryan Maves; Drake H Tilley; Tadeusz J Kochel; Wei-Mei Ching
Journal: Am J Trop Med Hyg Date: 2013-10-28 Impact factor: 2.345

9. Sensitivity and Specificity of a New Vertical Flow Rapid Diagnostic Test for the Serodiagnosis of Human Leptospirosis.

Authors: Cyrille Goarant; Pascale Bourhy; Eric D'Ortenzio; Sylvie Dartevelle; Carine Mauron; Marie-Estelle Soupé-Gilbert; Lilian Bruyère-Ostells; Ann-Claire Gourinat; Mathieu Picardeau; Faridabano Nato; Suzanne Chanteau
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