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Literature DB >> 17021239

Establishment of a gene expression system in Ochrobactrum anthropi.

Mohamed N Seleem¹, Mohammed Ali, Stephen M Boyle, Biswarup Mukhopadhyay, Sharon G Witonsky, Gerhardt G Schurig, Nammalwar Sriranganathan.

Abstract

Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.

Entities: Species

Mesh：

Year: 2006 PMID： 17021239 PMCID： PMC1610320 DOI： 10.1128/AEM.01446-06

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

16 in total

1. Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.

Authors: Ruifu Zhang; Zhongli Cui; Jiandong Jiang; Jian He; Xiangyang Gu; Shunpeng Li
Journal: Can J Microbiol Date: 2005-04 Impact factor: 2.419

2. Electroporation-mediated transformation of Aeromonas hydrophila.

Authors: Hu Fengqing; You Song
Journal: Plasmid Date: 2005-06-20 Impact factor: 3.466

3. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli.

Authors: P O Olins; C S Devine; S H Rangwala; K S Kavka
Journal: Gene Date: 1988-12-15 Impact factor: 3.688

4. Transformation of Brucella species with suicide and broad host-range plasmids.

Authors: J R McQuiston; G G Schurig; N Sriranganathan; S M Boyle
Journal: Methods Mol Biol Date: 1995

5. Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites.

Authors: E Katsivela; E R B Moore; D Maroukli; C Strömpl; D Pieper; N Kalogerakis
Journal: Biodegradation Date: 2005-03 Impact factor: 3.909

6. Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses.

Authors: R Vemulapalli; Y He; S M Boyle; N Sriranganathan; G G Schurig
Journal: Infect Immun Date: 2000-06 Impact factor: 3.441

3. Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2.

Authors: Amanda Pacholak; Natalia Burlaga; Ewa Kaczorek
Journal: Molecules Date: 2020-07-23 Impact factor: 4.411

3 in total

Establishment of a gene expression system in Ochrobactrum anthropi.

1. Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.

2. Electroporation-mediated transformation of Aeromonas hydrophila.

3. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli.

4. Transformation of Brucella species with suicide and broad host-range plasmids.

5. Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites.

6. Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses.

7. Construction of an efficient expression system for Agrobacterium tumefaciens based on the coliphage T5 promoter.

Review 8. Strategies for achieving high-level expression of genes in Escherichia coli.

9. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

10. Glutathione-dependent biotransformation of the fungicide chlorothalonil.

1. Tightly regulated expression vectors for Ochrobactrum anthropi.

2. Novel denitrifying bacterium Ochrobactrum anthropi YD50.2 tolerates high levels of reactive nitrogen oxides.

3. Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2.